CAJ | 학술논문

用鸡痘病毒载体表达马立克氏病病毒（MDV）糖蛋白B（gB）基因构建成重组鸡痘病毒（rFPV）。以MDV　GA或Md5和RBIB混合攻毒，在不同品种的鸡中评价rFPV－gB／R单价苗和与火鸡疱疹病毒（HVT）组成的二价苗的免疫保护效力。试验结果表明，MDV疫苗和FPV母源抗体阴性的SPF鸡和母源抗体阳性的商品鸡中，rFDV－gB／R均能提供免疫保护作用，且其中一种商品蛋鸡中rFPV－gB／R与HVT液氮苗或HVT冻干苗结合使用，均有显著的免疫协同保护作用。免疫学研究指出，rFPV－gB／R与常规疫苗一样，可显著地降低疫苗免疫攻毒鸡的病毒血症和羽囊排毒。
용계두병독재체표체마립극씨병병독（MDV）당단백B（gB）기인구건성중조계두병독（rFPV）。이MDV　GA혹Md5화RBIB혼합공독，재불동품충적계중평개rFPV－gB／R단개묘화여화계포진병독（HVT）조성적이개묘적면역보호효력。시험결과표명，MDV역묘화FPV모원항체음성적SPF계화모원항체양성적상품계중，rFDV－gB／R균능제공면역보호작용，차기중일충상품단계중rFPV－gB／R여HVT액담묘혹HVT동간묘결합사용，균유현저적면역협동보호작용。면역학연구지출，rFPV－gB／R여상규역묘일양，가현저지강저역묘면역공독계적병독혈증화우낭배독。
Recombinant fowlpox Virus （rFPV） was constructed to express glycoprotein B （gB） gene from CVI988／Rispens strain of Marek’s disease virus （MDV）．The rFPV－gB／R alone and in combination with herpesvirus of turkey （HVT） preparations were evaluated for their protective efficacy against challenge with different MDV strains in different chickens．The rFPV－gB／R alone induced protection comparable to that by HVT vaccines in both Ab-SPF chickens and Ab＋ production chickens．Significant protective synergism was observed in one of the two commercial production chickens when rFPV－gB／R was combined with HVT of either cell associated or cell free preparations．Immunogenesis studies showed that rFPV－gB／R，just like conventional vaccines，significantly reduced the level of viremia and feather follicle shedding with virus in vaccinated chickens．