CAJ | 학술논문

甜樱桃(Prunus avium)和酸樱桃(Prunus cerasus)分步微繁技术，此技术涵盖了微繁全过程所需的全套技术，包括外植体和培养基的制备方法，各个阶段的培养技术及培养基配方、试管苗炼苗和移栽等，采用这一技术体系，可在30周内完成从芽外植体建立到温室植株的全过程。此外，还提出了进一步改进该技术体系的建议，如调整总氮量及NO3-/NH4+比率，添加蔗糖以外的其他碳源，优化BAP和IBA浓度及配比等。
첨앵도(Prunus avium)화산앵도(Prunus cerasus)분보미번기술，차기술함개료미번전과정소수적전투기술，포괄외식체화배양기적제비방법，각개계단적배양기술급배양기배방、시관묘련묘화이재등，채용저일기술체계，가재30주내완성종아외식체건립도온실식주적전과정。차외，환제출료진일보개진해기술체계적건의，여조정총담량급NO3-/NH4+비솔，첨가자당이외적기타탄원，우화BAP화IBA농도급배비등。
This paper describes a step-by-step protocol of micropropagation for s weet cheery (Prunus avium) and sour cherry (P. cerasus). The protocol co vers the full information related to the whole process of micropropagation, including pr eparation of initial explants and media, a detailed account of the culture techn iques and medium formulae, and hardening and transplanting of the micro-plants. By using the protocol, complete plants of greenhouse acclimatization can be rege nerated within 30 weeks from bud explants. To further improve the protocol, some suggestions are offered, including adjusting the total nitrogen content and the ratio of NO3- to NH4+, supplementing sugars other than sucrose and optimi zing the concentrations of BAP and IBA and their balance.