CAJ | 학술논문

对中国棉铃虫单粒包埋核多角体病毒（HaSNPV）基因组中的HindⅢ-Ⅰ片段的序列进行分析，该片段全长7?501nt，包括10个开放阅读框：AcMNPVORF111的同源基因（Ac111），晚期表达因子2（lef-2）基因，病毒核壳结构蛋白p24基因、gp16基因、多角体包膜蛋白（polyhedronenvelopeprotein，pep）基因、AcMNPVORF63的同源基因（Ac63），38.7kD基因，晚期表达因子1（lef-1）基因，及两个特有基因HaSNPVorf591和HaSNPVorf435。基因组的排列比较分析发现与AcMNPV有较大区别。
대중국면령충단립포매핵다각체병독（HaSNPV）기인조중적HindⅢ-Ⅰ편단적서렬진행분석，해편단전장7?501nt，포괄10개개방열독광：AcMNPVORF111적동원기인（Ac111），만기표체인자2（lef-2）기인，병독핵각결구단백p24기인、gp16기인、다각체포막단백（polyhedronenvelopeprotein，pep）기인、AcMNPVORF63적동원기인（Ac63），38.7kD기인，만기표체인자1（lef-1）기인，급량개특유기인HaSNPVorf591화HaSNPVorf435。기인조적배렬비교분석발현여AcMNPV유교대구별。
In order to investigate the genomic organization of thesingle-nucleocapsid nucleopolyhedrovirus（SNPV） of Helicoverpa armigera（HaSNPV），the HindⅢ-Ⅰ fragment which is 7，501nt long and locates at map units（mu）12.3-17.9 of the viral genome was sequenced． The fragment contained ten open reading frames（ORFs）representing homologues of the AcMNPV orf 111 and orf 63，a lef-2 gene，a p24 gene，a gp16 gene，a pep gene，a 38． 7kD gene，a lef-1 gene and two unique genes named orf 591 and orf 435． Sequence alignment revealed that baculoviral P24，PEP and LEF-1 have some conserved motifs and gene structure as those observed in their homologues from other baculoviruses． The genomic arrangement of the ORFs in the HaSNPV HindⅢ-Ⅰ fragment is very different from the arrangement of their homologues in the genome of Autographa californica multiple nucleocapsid（M）NPV．