CAJ | 학술논문

用PEG经根部处理15d龄的山黧豆幼苗，取幼苗叶片为实验材料，测定过氧化氢酶(CAT)、过氧化物酶(POD)活性、过氧化氢(H2O2)和毒素(β-N-oxalyl-α，β-diaminopropionicacid，ODAP)的含量。结果表明，随着PEG处理时间的延长，POD和CAT活性降低，而H2O2和ODAP含量显著升高；在PEG处理液中加入二乙基二硫代氨基甲酸钠(diethyldithiocarbamate，DDC)和氨基三唑(aminotriazole，AT)后分别抑制和促进H2O2的产生，DDC可降低叶片中ODAP的含量，AT则使ODAP积累。由此我们推测，水分胁迫条件下活性氧代谢与ODAP的积累有关。
용PEG경근부처리15d령적산려두유묘，취유묘협편위실험재료，측정과양화경매(CAT)、과양화물매(POD)활성、과양화경(H2O2)화독소(β-N-oxalyl-α，β-diaminopropionicacid，ODAP)적함량。결과표명，수착PEG처리시간적연장，POD화CAT활성강저，이H2O2화ODAP함량현저승고；재PEG처리액중가입이을기이류대안기갑산납(diethyldithiocarbamate，DDC)화안기삼서(aminotriazole，AT)후분별억제화촉진H2O2적산생，DDC가강저협편중ODAP적함량，AT칙사ODAP적루。유차아문추측，수분협박조건하활성양대사여ODAP적적루유관。
Roots of fifteen-day-old grass peaseedlings were treated with PEGand then theleaves were used as material. Changes in activ-ities of catalase and peroxidase, and hydrogenperoxide and β-N-oxalyl-α, β-diaminopropi-onic acid (ODAP) contents during PEG treat-ment, were assayed. The results indicated thatduring PEG treatment, activities of peroxidaseand catalase decreased ( Fig. 3 ), and hydrogenperoxide ( Fig. 2 ) and ODAP content ( Fig. 1 )increased greatly. Diethyldithiocarbamate or a-minotriazole strongly decreased or increased re-spectively the H2O2 content ( Fig. 2 ). Whilethe accumulation of ODAP was decreasedmarkedly by diethyldithiocarbamate, it was in-creased significantly by aminotriazole ( Fig. 1 ).Therefore, we suggest that the accumulation ofODAP might be related to the increase in H2O2content under osmotic stress.