中国糖尿病杂志
中國糖尿病雜誌
중국당뇨병잡지
CHINESE JOURNAL OF DIABETES
2001年
1期
4-7
,共4页
2型糖尿病%磺脲类受体基因多态性%高胰岛素血症
2型糖尿病%磺脲類受體基因多態性%高胰島素血癥
2형당뇨병%광뇨류수체기인다태성%고이도소혈증
Sulfonylurea receptor gene polymorphism Hyperinsulinemia Type 2 diabetes
目的 了解磺脲类药物受体(SUR)基因多态性与中国汉族人糖尿病高危人群血清胰岛素浓度的关系。方法采用多聚酶链式反应-限制性酶切片段长度多态性分析技术(PCR-RFLP)对SUR基因第24内含子的多态性进行分析，采用放射免疫法(RIA)测定血清胰岛素浓度。结果 SUR基因第24内含子的“c”等位基因频率在206例有2型糖尿病家族史而彼此间无血缘关系的糖耐量试验正常人(A组)中较110例正常对照者(B组)显著升高(64% vs 54%，P=0.004)，且“cc”纯合子基因型的频率亦在此人群中显著升高(38% vs 24%，P=0.002)。A组人群中纯合子“cc”基因型者BMI(27.27±6.37 vs 24.99±3.43kg/m2，P＜0.05；27.27±6.37 vs 25.28±2.78kg/m2，P＜0.05)、空腹及口服75g葡萄糖后2小时血清胰岛素(15.52±10.72 vs 9.27±5.03U/ml,P＜0.01；15.52±10.72 vs 10.79±7.80U/ml,P＜0.05及76.41±54.02 vs 55.43±49.60U/ml,P＜0.01；76.41±54.02 vs 55.71±40.39,P＜0.05)、HOMA(Ri) (4.00±3.09 vs 2.79±1.32,P＜0.01；4.00±3.09 vs 2.82±2.94,P＜0.01)及ISI(-4.22±0.75 vs -3.69±0.57，P＜0.01；-4.22±0.75 vs -3.75±0.94,P＜0.01)均显著高于其它基因型者。结论 本研究表明SUR基因的缺陷可能导致胰岛素的高分泌，而后者可能是后继的体重增加及胰岛素敏感性降低的原因之一。本研究结果支持在糖尿病发病的自然病程中高胰岛素分泌为初始病因的假说。
目的 瞭解磺脲類藥物受體(SUR)基因多態性與中國漢族人糖尿病高危人群血清胰島素濃度的關繫。方法採用多聚酶鏈式反應-限製性酶切片段長度多態性分析技術(PCR-RFLP)對SUR基因第24內含子的多態性進行分析，採用放射免疫法(RIA)測定血清胰島素濃度。結果 SUR基因第24內含子的“c”等位基因頻率在206例有2型糖尿病傢族史而彼此間無血緣關繫的糖耐量試驗正常人(A組)中較110例正常對照者(B組)顯著升高(64% vs 54%，P=0.004)，且“cc”純閤子基因型的頻率亦在此人群中顯著升高(38% vs 24%，P=0.002)。A組人群中純閤子“cc”基因型者BMI(27.27±6.37 vs 24.99±3.43kg/m2，P＜0.05；27.27±6.37 vs 25.28±2.78kg/m2，P＜0.05)、空腹及口服75g葡萄糖後2小時血清胰島素(15.52±10.72 vs 9.27±5.03U/ml,P＜0.01；15.52±10.72 vs 10.79±7.80U/ml,P＜0.05及76.41±54.02 vs 55.43±49.60U/ml,P＜0.01；76.41±54.02 vs 55.71±40.39,P＜0.05)、HOMA(Ri) (4.00±3.09 vs 2.79±1.32,P＜0.01；4.00±3.09 vs 2.82±2.94,P＜0.01)及ISI(-4.22±0.75 vs -3.69±0.57，P＜0.01；-4.22±0.75 vs -3.75±0.94,P＜0.01)均顯著高于其它基因型者。結論 本研究錶明SUR基因的缺陷可能導緻胰島素的高分泌，而後者可能是後繼的體重增加及胰島素敏感性降低的原因之一。本研究結果支持在糖尿病髮病的自然病程中高胰島素分泌為初始病因的假說。
목적 료해광뇨류약물수체(SUR)기인다태성여중국한족인당뇨병고위인군혈청이도소농도적관계。방법채용다취매련식반응-한제성매절편단장도다태성분석기술(PCR-RFLP)대SUR기인제24내함자적다태성진행분석，채용방사면역법(RIA)측정혈청이도소농도。결과 SUR기인제24내함자적“c”등위기인빈솔재206례유2형당뇨병가족사이피차간무혈연관계적당내량시험정상인(A조)중교110례정상대조자(B조)현저승고(64% vs 54%，P=0.004)，차“cc”순합자기인형적빈솔역재차인군중현저승고(38% vs 24%，P=0.002)。A조인군중순합자“cc”기인형자BMI(27.27±6.37 vs 24.99±3.43kg/m2，P＜0.05；27.27±6.37 vs 25.28±2.78kg/m2，P＜0.05)、공복급구복75g포도당후2소시혈청이도소(15.52±10.72 vs 9.27±5.03U/ml,P＜0.01；15.52±10.72 vs 10.79±7.80U/ml,P＜0.05급76.41±54.02 vs 55.43±49.60U/ml,P＜0.01；76.41±54.02 vs 55.71±40.39,P＜0.05)、HOMA(Ri) (4.00±3.09 vs 2.79±1.32,P＜0.01；4.00±3.09 vs 2.82±2.94,P＜0.01)급ISI(-4.22±0.75 vs -3.69±0.57，P＜0.01；-4.22±0.75 vs -3.75±0.94,P＜0.01)균현저고우기타기인형자。결론 본연구표명SUR기인적결함가능도치이도소적고분비，이후자가능시후계적체중증가급이도소민감성강저적원인지일。본연구결과지지재당뇨병발병적자연병정중고이도소분비위초시병인적가설。
Objective A recent study has shown the association between a sulfonylurea receptor gene 1 (SUR1) variant and hyperinsulinemia in normal individuals from a high diabetes risk ethnic group,supporting the hypothesis that the primary insulin hypersecretion may be an antecedent of type 2 diabetes.Methods To test this hypothesis in Chinese population,we studied the allele and genotype distribution of the polymorphism at -3 position of intron 24 in SUR1 by PCR RFLP technique in 206 unrelated normal glucose tolerant subjects with strong family history of type 2 diabetes (group A) and 110 normal individuals without family history of diabetes (group B).Results The frequency of “-3c” allele and “-3cc” genotype of intron 24 in group A was significantly higher than that in group B (64% vs 54%, P =0 004 and 38% vs 24%, P =0.002 respectively).Moreover,in group A, those carrying “cc” genetype had a higher BMI (27 27±6 37 vs 24.99±3.43kg/m 2, P <0.05;27 27±6 37 vs 25.28±2.78kg/m 2, P <0.05),fasting insulin (15 52±10 72 vs 9.27±5.03U/ml, P <0.01;15 52±10 72 vs 10.79±7.80U/ml, P <0.05) and 2h insulin levels (76.41±54 02 vs 55.43±49.60U/ml, P <0.01;76 41±54 02 vs 55.71±40.39, P <0.05) as well as lower insulin sensitivity [HOMA(R i]: 4.00±3.09 vs 2.79±1.32, P <0.01; 4.00±3.09 vs 2.82±2.94, P <0.01) as compared with that in carriers of other genotypes (“ct” and “tt”).Conclusion This study suggested the possibility that the defect in SUR1 gene might contribute to the insulin hypersecretion which might be the cause of subsequent increased body weight and decreased insulin sensitivity.
