CAJ | 학술논문

比较我国沿海不同海域对虾白斑综合征杆状病毒三个分离株：即唐海分离株（渤海湾），宁波分离株（东海），深圳分离株（南海）的同源性。三个WSSV分离株基因组的限制性内切酶(SacI，HindIII，PstI) 酶切多态（RFLP）以及病毒结构蛋白图谱完全一致，证实造成我国从南至北对虾爆发性流行病的对虾白斑杆状病毒为同一种病毒。利用高保真Taq酶，分别以报道的日本对虾杆状病毒（RV-PJ＝PRDV），斑节对虾白斑综合征杆状病毒（WSBV＝PmNOBIII）基因组核酸片段特异性引物进行PCR扩增，结果均能从中国对虾白斑杆状病毒（WSSV）基因组中扩增得到相应大小的PCR产物，扩增产物序列分析表明中国对虾白斑杆状病毒（WSSV）与斑节对虾白斑综合征杆状病毒（WSBV＝PmNO BIII），日本对虾杆状病毒（RV－PJ＝PRDV）同源率分别为100％与97％，其结果为证实亚洲及太平洋地区对虾白斑综合征杆状病毒为同一种病毒或同一种病毒的不同株系提供了证据。
비교아국연해불동해역대하백반종합정간상병독삼개분리주：즉당해 분리주（발해만），저파분리주（동해），심수분리주（남해）적동원성。삼개WSSV분리주 기인조적한제성내절매(SacI，HindIII，PstI) 매절다태（RFLP）이급 병독결구단백도보완전일치，증실조성아국종남지북대하폭발성류행병적대하백반간상병독 위동일충병독。이용고보진Taq매，분별이보도적일본대하간상병독（RV-PJ＝PRDV），반절 대하백반종합정간상병독（WSBV＝PmNOBIII）기인조핵산편단특이성인물진행PCR확증，결 과균능종중국대하백반간상병독（WSSV）기인조중확증득도상응대소적PCR산물，확증산물 서렬분석표명중국대하백반간상병독（WSSV）여반절대하백반종합정간상병독（WSBV＝PmNO BIII），일본대하간상병독（RV－PJ＝PRDV）동원솔분별위100％여97％，기결과위증실아 주급태평양지구대하백반종합정간상병독위동일충병독혹동일충병독적불동주계제공료증거。
Homology of three WSSV isolates, which were sampled from r epresentative maritime space of China: Tanghai isolate (Bo Bay of China), Ningbo isolate (East China Sea), Shenzhen isolate (South China Sea) was compared. Both of the genome RFLP patterns and the characteristic structural proteins SDS-PAG E electrophero grams showed that they were quite same. It suggested that they were the same ki nd of WSSV virus that caused explosive epidemic diseases of shrimps (EEDS) throu ghout southern and northern China. The same large PCR products achieved when usi ng the PCR primers from RV－PJ＝PRDV (P. japonicus, Japan) and WSBV＝PmNOBII I(P.monodon Taiwan, China) respectively to amplify the genome from P.chine nsis (Tanghai, China) with high fidelity Taq Polymerase. The sequence identiti es of WSSV from P. chinensis with those from RV－PJ＝PRDV (P.japonicus, Japan) and WSBV＝PmNOBIII (P.monodon Taiwan, China) are 97% and 100% respect ively, the results provided additional evidence that WSSV reported in different parts of the Asian and Pacific regions maybe quite the same or just different va riants of the same virus.