CAJ | 학술논문

目的探讨细胞凋亡调控基因Bcl－2／Bax mRNA在子宫肌瘤中的表达及与ER、PR的关系。方法应用免疫组化法测定子宫肌瘤内ER、PR的含量(28例)。用RT-PCR法测定肌瘤内Bcl－2／Bax mRNA(21例)。以肌瘤邻近的正常子宫平滑肌作对照。结果子宫肌瘤内ER、PR，Bcl－2／Bax比值显著高于正常子宫平滑肌(P<0．05和P<0．01)；肌瘤ER、PR与Bcl－2／BaxmRNA比值呈正相关。结论细胞凋亡调控基因Bcl－2／Bax可能参与子宫肌瘤的发生；而雌、孕激素与ER、PR结合刺激肌瘤生长则可能与影响这一基因的表达有关。
목적 탐토세포조망조공기인Bcl－2／Bax mRNA재자궁기류중적표체급여ER、PR적관계。방법응용면역조화법측정자궁기류내ER、PR적함량(28례)。용RT-PCR법측정기류내Bcl－2／Bax mRNA(21례)。이기류린근적정상자궁평활기작대조。결과자궁기류내ER、PR，Bcl－2／Bax비치현저고우정상자궁평활기(P<0．05화P<0．01)；기류ER、PR여Bcl－2／BaxmRNA비치정정상관。결론세포조망조공기인Bcl－2／Bax가능삼여자궁기류적발생；이자、잉격소여ER、PR결합자격기류생장칙가능여영향저일기인적표체유관。
Objective To investigate the expression of apoptosis － controlled gene Bcl － 2/BaxmRNA in leiomyoma and it's relationship with ER、PR. Methods To measure ER, PR volume (28cases) in leiomyoma and the ratio of Bcl － 2/Bax mRNA (21 cases) by immunochemistry and RT －PCR, respectively, and use the myometrium as control. Results The content of ER, PR and the ra-tio of Bcl－ 2/Bax mRNA in leiomyoma were significantly higher than those in the myometrium (P＜ 0.05 and 0.01); there was positive correlation for ER, PR versus ratio of Bcl－ 2/Bax mRNA in leiomy-oma Conclusion Apoptosic－controlled gene Bcl － 2/Bax may play a role in the pathogenesis ofleiomyoma; the stimalating effect of estrogen and progesterone combining with ER, PR on leiomyomamay be related with their regulation of the expression of Bcl － 2/Bax mRNA.