CAJ | 학술논문

乳腺的淋巴管在不同生理时期的发育资料较少，研究旨在对不同发育时期的乳腺淋巴管进行定性定量分析。被分析的结构包括淋巴管内皮的面积(非核区)、内皮吞饮小泡的面积、内皮间开放连接与关闭连接的数目等。20只Wistar大鼠被分成四组：正常组、妊娠7天组、哺乳7天组和哺乳停止后7天组，每组各5只。动物经主动脉灌流生理盐水后，然后灌注20～30毫升墨汁以区别血管与淋巴管。乳腺组织标本经处理后，应用光镜、透射电镜及图像分析等方法对其进行了有关研究。乳腺的淋巴管可分为三类：叶间淋巴管、小叶间淋巴管和小叶内淋巴管(仅见于哺乳7天组)。淋巴管内皮间开放连接的数目占连接总数的百分比在各组分别为12.71％(正常组)、28％(妊娠7天组)、37.61％(哺乳7天组)和8.15％(哺乳停止后7天组)。淋巴管内皮吞饮小泡的面积与内皮总面积(非核区)的比在各组分别为4.2％(正常组)、1.9％(妊娠7天组)、0.9％(哺乳7天组)和2.7％(哺乳停止后7天组)。研究结果显示：在哺乳7天组和妊娠7天组，开放连接为淋巴管运输的主要通道；而在正常组，内皮吞饮小泡在运输过程中起重要作用。
유선적림파관재불동생리시기적발육자료교소，연구지재대불동발육시기적유선림파관진행정성정량분석。피분석적결구포괄림파관내피적면적(비핵구)、내피탄음소포적면적、내피간개방련접여관폐련접적수목등。20지Wistar대서피분성사조：정상조、임신7천조、포유7천조화포유정지후7천조，매조각5지。동물경주동맥관류생리염수후，연후관주20～30호승묵즙이구별혈관여림파관。유선조직표본경처리후，응용광경、투사전경급도상분석등방법대기진행료유관연구。유선적림파관가분위삼류：협간림파관、소협간림파관화소협내림파관(부견우포유7천조)。림파관내피간개방련접적수목점련접총수적백분비재각조분별위12.71％(정상조)、28％(임신7천조)、37.61％(포유7천조)화8.15％(포유정지후7천조)。림파관내피탄음소포적면적여내피총면적(비핵구)적비재각조분별위4.2％(정상조)、1.9％(임신7천조)、0.9％(포유7천조)화2.7％(포유정지후7천조)。연구결과현시：재포유7천조화임신7천조，개방련접위림파관운수적주요통도；이재정상조，내피탄음소포재운수과정중기중요작용。
To qualitatively and quantitatively examine lymphatics of the rnammary gland during different physiological periods in Wistar rats. Methods: The structures analyzed included the area of the endothelia(non-nuclear area), area of the endothelial vesicles, the number and types of open and dose junctions. Twenty Wistar rats were divided into 4 groups: normal, pregnancy on day 7,lactating on day 7, post-weaning on day 7 groups(n ＝ 5). The animals were perfused with 0.9%saline and then with 20 ～ 30 ml India ink through the aorta to distinguish blood vessels from lymphatics The mammary gland tissue was treated with routine LM and TEM procedures. The related research were done by LM, TEM and morphometry. Results: The initial lymphatics of the mammary gland were classified into 3 categories: (1) interlobar lymphatic, (2) interlobular lymphatic and (3) intralobular lymphatic (only found in lactating group). The ratio between the open and the totaljunctions of the lymphatics was 12. 7196 (normal), 2896 (pregnancy on day 7), 37. 6196(lactating on day 7), and 8. 159% (post-weaning on day 7). The area ratio between the endothelial vesicles and the total endothelia in each group was 4.2 96 (normal), 1.9 96 (pregnancy on day 7), 0.996 (lactating on day 7), and 2.796 (post-weaning on day 7). Conclusion: During lactating and pregnancy on day 7, open junctions are the major route for the transport; while in the normal group,the endothelial vesicular transport plays an important role.