CAJ | 학술논문

为探讨颌面部火器伤损伤组织免疫动态变化,采用免疫组化、核酸杂交方法对颌面部火器伤损伤组织中白介素2（IL-2）的表达情况及其发生的细胞学和分子学机制进行了研究。发现伤后1天内IL-2蛋白产物和IL-2mRNA表达呈“一过性”升高，但此后较前明显下降，并且以组织间接损伤区表现尤为显著。提示颌面部火器伤可导致局部损伤组织IL-2产生低下，从而引起损伤组织局部组织细胞免疫抑制。这种抑制作用与IL-2mRNA表达下调有关。颌面部火器伤投射物的间接致伤作用是造成IL-2mRNA表达受抑制的主要因素。
위탐토합면부화기상손상조직면역동태변화,채용면역조화、핵산잡교방법대합면부화기상손상조직중백개소2（IL-2）적표체정황급기발생적세포학화분자학궤제진행료연구。발현상후1천내IL-2단백산물화IL-2mRNA표체정“일과성”승고，단차후교전명현하강，병차이조직간접손상구표현우위현저。제시합면부화기상가도치국부손상조직IL-2산생저하，종이인기손상조직국부조직세포면역억제。저충억제작용여IL-2mRNA표체하조유관。합면부화기상투사물적간접치상작용시조성IL-2mRNA표체수억제적주요인소。
To investigate the temporal variation of cell-mediated immunity in injured tissue of maxillofacial firearm trauma. Cells expressing messenger RNA(mRNA) for IL-2 were demonstrated by dot blotting and in situ hybrization in injured tissue of maxillofacial trauma inflicted by bullet, in conjunction with immunohistochemistry for IL-2 in normal and postinjury conditions. It was found that, apart from a transient eleratim in level within 24h post injury, both IL-2 production and IL-2 mRNA expression were consistently suppressed in cells at the site of the wound compared with the normal. The changes in the tissues of indirect injury by the projectile were significant. These results indicate that the cell-mediated immunity was suppressed in maxillofacial firearm trauma and the principal cellular abnormalities that resulted in altered T cell activation and IL-2 production postinjury was downregulation of mRNA before IL-2 gene transcription. It also suggested that the indirect injury by projectile contribute mainly to the depression of cell-mediated immunity.