科学通报（英文版）
科學通報（英文版）
과학통보（영문판）
CHINESE SCIENCE BULLETIN
2000年
6期
516-520
,共5页
fragile X mental retardation protein%G/T mismatch-specific DNA thymine glycosylase%yeast two-hybrid%protein/protein interaction
Fragile X syndrome is the most common form of inherited mental retardation disease,resulting from absent of expression of its disease gene FMR1.To study the function of the fragile X mental retardation protein (FMRP) through protein/protein interaction,a mouse embryo cDNA library was screened by the yeast two-hybrid system.A clone was found to interact specifically with FMRP.The cDNA of this clone ( Genbank accession number af102875 ) encoded a protein highly homologous to human G/T mismatch-specific DNA thymine glycosylase ( hTDG ).Interactions between various alternatively spliced FMRP isoforms and a series of mTDG deletion proteins were further studied in the yeast two-hybrid system and their interaction amino acid regions were determined.Interaction between FMRP and TDG existed inside exon 13 of FMRP ( amino acid residue 397-425 ) and around amino acid residue 122-346 of TDG.These results will be helpful to the study of the biological role of FMRP.
