CAJ | 학술논문

在4种不同的培养程序中应用了几种处理方法，并对其在诱导水稻原生质体起源的愈伤组织再生植株中的效果进行了比较。直接将愈伤组织从含有2，4-D的增殖培养基转移到含有BA和NAA的植株再生培养基上培养，只能得到少量的弱苗(第1种程序)。在增殖培养基中添加ABA诱导了结节状的愈伤组织形成，使愈伤组织的植株再生能力明显加强(第2种程序)；而在植株再生培养基中添加ABA则使愈伤组织变得紧结并形成生长受抑制的不定芽，当这些愈伤组织被转移到不含ABA的生长培养基后，不定芽开始快速生长(第3种程序)。先将愈伤组织培养在含有ABA的增殖培养基上，然后相继转移到含有ABA的植株再生培养基和生长培养基上，可以取得大量健壮的再生苗(第4种程序)。统计结果显示，第2和第3种程序的培养效果比第1种程序要好，而第4种程序的培养效果则比其他程序好得多。
재4충불동적배양정서중응용료궤충처리방법，병대기재유도수도원생질체기원적유상조직재생식주중적효과진행료비교。직접장유상조직종함유2，4-D적증식배양기전이도함유BA화NAA적식주재생배양기상배양，지능득도소량적약묘(제1충정서)。재증식배양기중첨가ABA유도료결절상적유상조직형성，사유상조직적식주재생능력명현가강(제2충정서)；이재식주재생배양기중첨가ABA칙사유상조직변득긴결병형성생장수억제적불정아，당저사유상조직피전이도불함ABA적생장배양기후，불정아개시쾌속생장(제3충정서)。선장유상조직배양재함유ABA적증식배양기상，연후상계전이도함유ABA적식주재생배양기화생장배양기상，가이취득대량건장적재생묘(제4충정서)。통계결과현시，제2화제3충정서적배양효과비제1충정서요호，이제4충정서적배양효과칙비기타정서호득다。
Four different culture procedures combined with several treating methods werecompared for their effects on regeneration of plants from protoplast-derived calluses of rice. Only a small number of weak plants could be regenerated when the calluses were transferred and cultured directly from a proliferation medium containing 2, 4-D to a plant regeneration medium containing BA and NAA (procedure 1). Addition of ABA to the proliferation medium induced the formation of nodular calluses with enhanced regeneration potential (procedure 2 ), whileaddition of ABA to the regeneration medium resulted in the formation of compact calluses withsuppressed adventitious buds, which would grow fast upon transfer to a growth medium free of ABA (procedure 3 ). By Culturing of the calluses consequently on ABA supplementedproliferation medium, ABA supplemented regeneration medium and the growth medium(procedure 4), large number of more healthy plants was obtained. Statistical test indicates thatprocedure 2 and 3 were mumore efficient than procedure l, while procedure 4 was the mostefficient for plant regeneration.