中草药
中草藥
중초약
CHINESE TRADITIONAL AND HERBAL DRUGS
2003年
2期
166-168
,共3页
韩健%戴均贵%崔亚君%占纪勋%郭洪祝%果德安
韓健%戴均貴%崔亞君%佔紀勛%郭洪祝%果德安
한건%대균귀%최아군%점기훈%곽홍축%과덕안
长春花%银杏%生物转化%青蒿素%悬浮细胞
長春花%銀杏%生物轉化%青蒿素%懸浮細胞
장춘화%은행%생물전화%청호소%현부세포
Catharanthus roseus (L.) G. Don%Ginkgo biloba L.%biotransformation%artemisinin%cell suspension culture
目的对抗疟药物青蒿素(Ⅰ)进行了生物转化研究.方法利用长春花及银杏植物细胞悬浮培养细胞进行生物转化.用硅胶柱色谱进行产物的分离,波谱方法鉴定产物的结构.结果此两种植物悬浮细胞体系均能将青蒿素转化成3α-羟基去氧青蒿素(Ⅱ).结论此两种植物悬浮细胞体系均能有效转化青蒿素.
目的對抗瘧藥物青蒿素(Ⅰ)進行瞭生物轉化研究.方法利用長春花及銀杏植物細胞懸浮培養細胞進行生物轉化.用硅膠柱色譜進行產物的分離,波譜方法鑒定產物的結構.結果此兩種植物懸浮細胞體繫均能將青蒿素轉化成3α-羥基去氧青蒿素(Ⅱ).結論此兩種植物懸浮細胞體繫均能有效轉化青蒿素.
목적대항학약물청호소(Ⅰ)진행료생물전화연구.방법이용장춘화급은행식물세포현부배양세포진행생물전화.용규효주색보진행산물적분리,파보방법감정산물적결구.결과차량충식물현부세포체계균능장청호소전화성3α-간기거양청호소(Ⅱ).결론차량충식물현부세포체계균능유효전화청호소.
Object To investigate the biotransformation of the antimalarial compound artemisinin( Ⅰ ) by Catharanthus roseus and Ginkgo biloba cell suspension cultures. Methods Plant tissue culture technology was employed. The product was isolated on silica gel column chromatography and its structure was elucidated by spectroscopic evidence. Results One product was obtained and its structure was characterized as 3α- hydroxydeoxyartemisinin ( Ⅱ ). Conclusion Both of C. roseus and G. biloba cell suspension cultures can bioconvert artemisinin.
