CAJ | 학술논문

钙调蛋白依赖的蛋白激酶(CaMK)是一类分布广泛的丝/苏氨酸蛋白激酶家族,在钙离子和钙调蛋白存在的条件下发生自磷酸化而被激活,在细胞内对于钙信号的传递具有重要的介导作用.近年来的研究表明CaMKⅡ是参与调节卵母细胞减数分裂的重要分子,在卵母细胞成熟、极体排放、受精和活化等过程中发挥作用.CaMKⅡ作为Ca2+的下游信号分子,在受精后促进成熟促进因子(MPF)和细胞静止因子(CSF)的失活,并调节纺锤体微管的组装和中心体的复制过程.虽然CaMKⅡ在减数分裂中的作用广泛而关键,但目前的研究主要集中于低等动物和小鼠,今后有待进一步阐明该蛋白激酶在其他哺乳动物中的作用和调节机制.
개조단백의뢰적단백격매(CaMK)시일류분포엄범적사/소안산단백격매가족,재개리자화개조단백존재적조건하발생자린산화이피격활,재세포내대우개신호적전체구유중요적개도작용.근년래적연구표명CaMKⅡ시삼여조절란모세포감수분렬적중요분자,재란모세포성숙、겁체배방、수정화활화등과정중발휘작용.CaMKⅡ작위Ca2+적하유신호분자,재수정후촉진성숙촉진인자(MPF)화세포정지인자(CSF)적실활,병조절방추체미관적조장화중심체적복제과정.수연CaMKⅡ재감수분렬중적작용엄범이관건,단목전적연구주요집중우저등동물화소서,금후유대진일보천명해단백격매재기타포유동물중적작용화조절궤제.
Calmodulin-dependent protein kinase (CaMK), activated by auto-phosphorylation at the presence of calcium and calmodulin, is widely distributed in eukaryotes. CaMKs are important mediators of calcium signal in eukaryotes. Recent researches have suggested that CaMKⅡ is involved in the regulation of meiotic cell cycle of oocytes. It plays functional roles in meiotic maturation, polar body extrusion, fertilization and egg activation. As one of the down-stream signaling molecules of calcium, CaMKⅡ facilitates the inactivation of maturation promoting factor (MPF) and cytostatic factor (CSF) following fertilization, as well as the spindle microtubule organization and centrosome duplication. Although the functions of CaMKⅡ in oocyte meiosis are versatile and essential, the present results are primarily obtained from low vertebrates and mouse. In future studies, the function and regulation of this kinase in other mammals should be stressed.