CAJ | 학술논문

运用快速扣除杂交(RaSH)方法构建了水稻氮饥饿诱导的cDNA文库.从该文库获得了一个cDNA克隆OsNSI1 (Oryza sativa nitrogen starvation- inducible 1).该全长cDNA编码577个氨基酸,蛋白分子量为61.2 kD.推测得出的氨基酸序列与其他物种的糖转运体有很高的同源性.水合性分析表明OsNSI1 包含有12 个跨膜区域和一个中心亲水环.这些数据提示OsNSI1 是一个糖转运体蛋白.Southern 印迹分析表明OsNSI1 是一个单拷贝基因.Northern印迹分析表明OsNSI1 主要在叶及根中表达,氮、磷饥饿能强烈诱导其表达增强.
운용쾌속구제잡교(RaSH)방법구건료수도담기아유도적cDNA문고.종해문고획득료일개cDNA극륭OsNSI1 (Oryza sativa nitrogen starvation- inducible 1).해전장cDNA편마577개안기산,단백분자량위61.2 kD.추측득출적안기산서렬여기타물충적당전운체유흔고적동원성.수합성분석표명OsNSI1 포함유12 개과막구역화일개중심친수배.저사수거제시OsNSI1 시일개당전운체단백.Southern 인적분석표명OsNSI1 시일개단고패기인.Northern인적분석표명OsNSI1 주요재협급근중표체,담、린기아능강렬유도기표체증강.
A cDNA library from nitrogen-starved rice (Oryza sativa L.) was constructed using RaSH procedure.A cDNA clone,OsNSI1 (Oryza sativa nitrogen starvation-inducible 1) was isolated from this library.The full-length cDNA encodes a protein of 577 amino acids with a predicted molecular weight of 61.2 kD.The deduced amino acid sequence of OsNSI1 shows high homology to sugar transporters from other species.Hydropathy analysis revealed that OsNSI1 protein composed of 12 transmembrane domains and a central hydrophilic loop.These data suggest that OsNSI1 encodes a sugar transporter.Genomic Southern blot analysis showed that OsNSI1 may be a single-copy gene in rice genome.Northern blot analysis revealed that OsNSI1 was mainly expressed in leaves and roots,and upregulated by N- and P-starvation.