CAJ | 학술논문

利用15%～25%丙烯酰胺梯度凝胶的SDS-PAGE分析法可将水稻种子贮藏谷蛋白分离为3个酸性(α)亚基和3个碱性(β)亚基.通过调整两性电解质比例对现有等电点聚焦电泳分析法进行改良,可将谷蛋白酸性亚基和碱性亚基分别分划为13和14条多肽带.将上述两种方法结合起来的双向电泳分析法可以高清晰度地离析谷蛋白并获得单一多肽.此改良的电泳分析系统有助于确定水稻谷蛋白变异及谷蛋白的生化研究.
이용15%～25%병희선알제도응효적SDS-PAGE분석법가장수도충자저장곡단백분리위3개산성(α)아기화3개감성(β)아기.통과조정량성전해질비례대현유등전점취초전영분석법진행개량,가장곡단백산성아기화감성아기분별분화위13화14조다태대.장상술량충방법결합기래적쌍향전영분석법가이고청석도지리석곡단백병획득단일다태.차개량적전영분석계통유조우학정수도곡단백변이급곡단백적생화연구.
Rice seed storage glutelin could be separated into three acidic (α-) and three basic (β-) subunit components by SDS-PAGE using 15%～25% gradient acrylamide gel. More than 13 acidic and 14 basic bands may be distinguished with an improved iso-electric focus electrophoresis system by adjusting Ampholine ratios. Combine these two sensitive electrophoresis systems resulting in two-dimensional electrophoresis giving high resolution of glutelin leading the yield of single polypeptide. The improved electrophoresis system is helpful in identifying rice glutelin variation and for further studies of glutelin biochemistry.