CAJ | 학술논문

作者研究开发出一种基于分析中间响应模式确定悬浮培养诱导子作用位点的新方法.研究结果表明,一个诱导子的作用位点存在于浓度变化方向相反的相邻两个中间代谢物之间;该方法的有效性在悬浮培养南方红豆杉(Taxus chinensis(Pilg.)Rehd.var.mairei(Lemee et Levl.)Cheng et L. K.Fu)生物合成紫杉醇过程中得以证实;经确定,甲基茉莉酮酸、硝酸根和柠檬酸铵的作用位点在baccatinⅢ至10-去乙酰基紫杉醇之间;水杉酸、花生四烯酸的作用位点存在3种可能性,即增强10-去乙酰基紫杉醇的合成、防止紫杉醇和cephalomannine的降解.该方法对指导诱导子配伍优化紫杉醇生产具有指导意义.
작자연구개발출일충기우분석중간향응모식학정현부배양유도자작용위점적신방법.연구결과표명,일개유도자적작용위점존재우농도변화방향상반적상린량개중간대사물지간;해방법적유효성재현부배양남방홍두삼(Taxus chinensis(Pilg.)Rehd.var.mairei(Lemee et Levl.)Cheng et L. K.Fu)생물합성자삼순과정중득이증실;경학정,갑기말리동산、초산근화저몽산안적작용위점재baccatinⅢ지10-거을선기자삼순지간;수삼산、화생사희산적작용위점존재3충가능성,즉증강10-거을선기자삼순적합성、방지자삼순화cephalomannine적강해.해방법대지도유도자배오우화자삼순생산구유지도의의.
A new method was developed to determine the acting paths of elicitors based on the analysis of intermediate response mode in suspension cultures. It was found that the acting path of an elicitor,defined as the biosynthesis step whose rate was significantly enhanced upon the addition of the elicitor,was located between two adjacent intermediates whose concentrations changed in opposite directions.The validity of this method was verified in the biosynthesis of taxol from suspension cultures of Taxus chinensis (Pilg.) Rehd. var. mairei(Lemee et Levl.) Cheng et L. K. Fu. The acting paths of methyl jasmonate,silver nitrate and ammonium citrate were determined to be located at the step from baccatin Ⅲ to 10-deacyl taxol. There are three possibilities for salicylic acid and arachidonic acid: (1) improving the biosynthesis of 10-deactyl baccatin Ⅲ (10-DAB), (2) inhibiting the degradation of taxol and (3) inhibiting the degradation of cephalomannine. But it is difficult to elucidate the exact acting paths of these two elicitors based on the present method.