分析测试学报
分析測試學報
분석측시학보
JOURNAL OF INSTRUMENTAL ANALYSIS
2009年
7期
799-803
,共5页
分子印迹聚合物膜%对羟基苯甲酸酯类%方波伏安法%电化学传感器
分子印跡聚閤物膜%對羥基苯甲痠酯類%方波伏安法%電化學傳感器
분자인적취합물막%대간기분갑산지류%방파복안법%전화학전감기
molecularly imprinted polymer membrane%paraben%square wave voltammetry%electrochemical sensor
利用分子印迹技术,以对羟基苯甲酸乙酯为模板分子,甲基丙烯酸为单体,在玻碳电极表面原位聚合分子印迹聚合物敏感膜.采用方波伏安法对对羟基苯甲酸乙酯在该印迹电极上的电化学行为进行了研究,当响应时间为15 min时,0.95 V(vs SCE)处的峰电流与对羟基苯甲酸乙酯的浓度在2.0×10-6 ～2.0×10-4 mol/L范围内呈线性关系,检出限(S/N=3)为1.0 μmol/L,而相同条件下,对羟基苯甲酸乙酯在控制电极上的响应非常小并对浓度变化不灵敏;同时在印迹电极上氧化峰电位较在裸电极上发生了微弱正移.同一支印迹电极对对羟基苯甲酸乙酯响应值的RSD为4.3%(n=10).该印迹电极对尼泊金酯类具有良好的选择性,对对羟基苯甲酸甲酯、丙酯以及丁酯的选择性系数分别为1.89、1.70和2.01;对结构相似的苯酚、对羟基苯甲酸、对氨基苯甲酸等响应不灵敏,对结构差异较大的如维生素C等几乎无响应.用该分子印迹电极对实际样品进行分析,加标回收率大于90%.
利用分子印跡技術,以對羥基苯甲痠乙酯為模闆分子,甲基丙烯痠為單體,在玻碳電極錶麵原位聚閤分子印跡聚閤物敏感膜.採用方波伏安法對對羥基苯甲痠乙酯在該印跡電極上的電化學行為進行瞭研究,噹響應時間為15 min時,0.95 V(vs SCE)處的峰電流與對羥基苯甲痠乙酯的濃度在2.0×10-6 ～2.0×10-4 mol/L範圍內呈線性關繫,檢齣限(S/N=3)為1.0 μmol/L,而相同條件下,對羥基苯甲痠乙酯在控製電極上的響應非常小併對濃度變化不靈敏;同時在印跡電極上氧化峰電位較在裸電極上髮生瞭微弱正移.同一支印跡電極對對羥基苯甲痠乙酯響應值的RSD為4.3%(n=10).該印跡電極對尼泊金酯類具有良好的選擇性,對對羥基苯甲痠甲酯、丙酯以及丁酯的選擇性繫數分彆為1.89、1.70和2.01;對結構相似的苯酚、對羥基苯甲痠、對氨基苯甲痠等響應不靈敏,對結構差異較大的如維生素C等幾乎無響應.用該分子印跡電極對實際樣品進行分析,加標迴收率大于90%.
이용분자인적기술,이대간기분갑산을지위모판분자,갑기병희산위단체,재파탄전겁표면원위취합분자인적취합물민감막.채용방파복안법대대간기분갑산을지재해인적전겁상적전화학행위진행료연구,당향응시간위15 min시,0.95 V(vs SCE)처적봉전류여대간기분갑산을지적농도재2.0×10-6 ～2.0×10-4 mol/L범위내정선성관계,검출한(S/N=3)위1.0 μmol/L,이상동조건하,대간기분갑산을지재공제전겁상적향응비상소병대농도변화불령민;동시재인적전겁상양화봉전위교재라전겁상발생료미약정이.동일지인적전겁대대간기분갑산을지향응치적RSD위4.3%(n=10).해인적전겁대니박금지류구유량호적선택성,대대간기분갑산갑지、병지이급정지적선택성계수분별위1.89、1.70화2.01;대결구상사적분분、대간기분갑산、대안기분갑산등향응불령민,대결구차이교대적여유생소C등궤호무향응.용해분자인적전겁대실제양품진행분석,가표회수솔대우90%.
A molecularly imprinted polymers(MIPs) was synthesized by using ethyl paraben(EP) as template and methacrylic acid as functional monomer,and a molecularly imprinted electrode was constructed on the surface of a glassy carbon electrode for determination of parabens,such as EP,methyl paraben(MP),butyl paraben(BP) and propyl paraben(PP).Electrochemical oxidation behaviors of EP on the imprinted electrode were investigated by square wave voltammetric method.When using the incubation time of 15 min,the peak currents at oxidation potential of 0.95 V(vs SCE) were proportional to the concentration of EP in the range of 2.0×10-6 to 2.0×10-4 mol/L with a detection limit of 1.0 μmol/L(S/N=3).The RSD(n=10) of the imprinted electrode for peak current was 4.3%(n=10).Under the same conditions,the extremely small responses of the control electrode were observed and independent of the analyte concentration.The oxidation potential of EP recorded with imprinted electrode was a slight positive shift comparing with that of the bare electrode.The imprinted electrode also displayed good specific binding MP,PP and BP,and their selective coefficients of imprinted electrode were 1.89,1.70 and 2.01,respectively.Structural analogs,such as phenol,p-hydroxybenzoic acid and p-aminobenzoic acid,only small responses electrode were observed.Moreover,structural unanalog vitamin C had almost no response.The imprinted electrode was used for analysis of parabens in actual samples with the spiked recoveries more than 90%.The molecularly imprinted polymer electrode showed good sensitivity and selectivity,and was simple to construct and operate.
