CAJ | 학술논문

目的:探讨局灶性脑缺血再灌注后应用白术多糖治疗对神经功能的保护作用.方法:采用局灶性脑缺血再灌注模型,28只雄性Sprague Dawley大鼠随机分为对照组、再灌注组、蒸馏水组及白术多糖组.再灌注24h进行神经功能缺损评分,取缺血区组织,分别检测其水分、Na+、K+含量,同时观察病理形态学改变情况.结果:白术多糖组神经功能缺损评分明显低于再灌注组及蒸馏水组(P<0.05);白术多糖组神经元损伤数量明显少于再灌注组及蒸馏水组(P<0.01);白术多糖组水分、Na+(含量显著低于再灌注组及蒸馏水组(P<0.01),K+含量显著高于再灌注组及蒸馏水组(P<0.01).结论:白术多糖能减轻局灶性脑缺血再灌注后脑水肿的程度,减少神经细胞的受损,改善神经功能缺损状态,而起神经保护作用.
목적:탐토국조성뇌결혈재관주후응용백술다당치료대신경공능적보호작용.방법:채용국조성뇌결혈재관주모형,28지웅성Sprague Dawley대서수궤분위대조조、재관주조、증류수조급백술다당조.재관주24h진행신경공능결손평분,취결혈구조직,분별검측기수분、Na+、K+함량,동시관찰병리형태학개변정황.결과:백술다당조신경공능결손평분명현저우재관주조급증류수조(P<0.05);백술다당조신경원손상수량명현소우재관주조급증류수조(P<0.01);백술다당조수분、Na+(함량현저저우재관주조급증류수조(P<0.01),K+함량현저고우재관주조급증류수조(P<0.01).결론:백술다당능감경국조성뇌결혈재관주후뇌수종적정도,감소신경세포적수손,개선신경공능결손상태,이기신경보호작용.
Objective: To study the neuroprotective effect of polysaccharide ofAtractylodes macrocephala Koidz on focal cerebral ischemia reperfusion in rats. Methods: Rats were subjected to 1 h middle cerebral artery occlusion (MCAO) using an intraluminal nylon filament followed by 24 h reperfusion. Twenty-eight male Sprague Dawley rats were randomly divided into four groups: sham group, reperfusion group, distilled water group and polysaccharide ofAtractylodes macrocephala Koidz group. The neurological deficit score was determined and the contents of water and total sodium, potassium were detected and pathological changes were observed in the ischemic brain tissues. Results and the neurological deficit scores in Atractylodes macrocephala Koidz group were significantly lower than those in reperfusion group and distilled water group (P<0.05). The injured neurons in Atractylodes macrocephala Koidz group were significantly fewer than those in reperfusion group and distilled water group (P <0.01). The contents of water and total sodium in Polysaccharide of Atractylodes macrocephala Koidz group were significantly lower than those in reperfusion group and distilled water group (P<0.01). The total potassium in polysaccharide of Atractylodes macrocephala Koidz group was significantly higher than that in reperfusion group and distilled water group (P < 0.01). Conclusion: polysaccharide of Atractylodes macrocephala Koidz can show neuroprotective effect on focal cerebral ischemia reperfusion via alleviating brain edema, decreasing injured neurons and improving neurologic impairment.