CAJ | 학술논문

通过H-E.染色法和免疫组化方法,对棕色田鼠视网膜结构及神经元特异性烯醇化酶的分布特性进行研究.结果表明,棕色田鼠的视网膜结构呈典型的脊椎动物视网膜结构类型,由外向内分别为色素上皮、视锥视杆层、外核层、外网层、内核层、内网层、节细胞层、神经纤维层.视网膜中神经元特异性烯醇化酶阳性分布在内网层、内核层、靠近内核层的外网层区、节细胞层、神经纤维层,其中内网层阳性最强,有4个阳性亚层.视锥视杆层和外核层没有神经元特异性烯醇化酶分布,这可能与感光细胞的分泌有关.
통과H-E.염색법화면역조화방법,대종색전서시망막결구급신경원특이성희순화매적분포특성진행연구.결과표명,종색전서적시망막결구정전형적척추동물시망막결구류형,유외향내분별위색소상피、시추시간층、외핵층、외망층、내핵층、내망층、절세포층、신경섬유층.시망막중신경원특이성희순화매양성분포재내망층、내핵층、고근내핵층적외망층구、절세포층、신경섬유층,기중내망층양성최강,유4개양성아층.시추시간층화외핵층몰유신경원특이성희순화매분포,저가능여감광세포적분비유관.
Retinal structure and characteristics of Lasiopodomys mandarinus were studied by using H-E.staining and immunohistochemical method.The results showed that the structure of retina in Lasiopodomys mandarinus exhibited typical structure of vertebrates,including retinal pigment epitheli-um (RPE),rod cone layer(PRL),outer nuclear layer(ONL),outer plexiform layer (OPL),inner nuclear layer (INL),inner plexiform layer (IPL),ganglion cell layer (GCL),and nerve fiber layer (NFL) from outside to inside.The distribution of positive NSE in the retina localized mainly in IPL,INL,the area near INL in OPL,GCL,and NFL,of which IPL was the most positive,and four positive sub-layers could be seen.Distribution of positive NSE was not found in PRL and ONL,which might be related to the secretion of photoreceptor celia.