吉林大学学报（理学版）
吉林大學學報（理學版）
길림대학학보（이학판）
JOURNAL OF JILIN UNIVERSITY(SCIENCE EDITION)
2010年
2期
333-338
,共6页
李鸿梅%李玉扩%李红%倪鹏%宋欣%徐力
李鴻梅%李玉擴%李紅%倪鵬%宋訢%徐力
리홍매%리옥확%리홍%예붕%송흔%서력
玉米源活性肽%分离纯化%2,2-二苯基-1-苦基苯肼(DPPH)自由基%清除作用
玉米源活性肽%分離純化%2,2-二苯基-1-苦基苯肼(DPPH)自由基%清除作用
옥미원활성태%분리순화%2,2-이분기-1-고기분정(DPPH)자유기%청제작용
corn active peptide%separation and purification%2,2-diphenyl-1-picrylhydrazyl (DPPH) radical%scavenging effect
以脱脂醇溶蛋白为底物进行酶水解反应,获得了玉米源活性肽组分,经葡聚糖凝胶和离子交换等方法对产物进一步分离纯化. 以α-生育酚为阳性对照,研究了活性肽及其分离纯化组分对2,2-二苯基-1-苦基苯肼(DPPH)自由基的清除活性及其浓度的关系. 结果表明: 活性肽及其分离纯化组分均具有清除DPPH自由基的活性;经CM-纤维素弱阳离子交换层析分离所得组分2对DPPH自由基的清除活性最强,且清除效果与其浓度及作用时间呈正相关,分子量主要集中在400～700.
以脫脂醇溶蛋白為底物進行酶水解反應,穫得瞭玉米源活性肽組分,經葡聚糖凝膠和離子交換等方法對產物進一步分離純化. 以α-生育酚為暘性對照,研究瞭活性肽及其分離純化組分對2,2-二苯基-1-苦基苯肼(DPPH)自由基的清除活性及其濃度的關繫. 結果錶明: 活性肽及其分離純化組分均具有清除DPPH自由基的活性;經CM-纖維素弱暘離子交換層析分離所得組分2對DPPH自由基的清除活性最彊,且清除效果與其濃度及作用時間呈正相關,分子量主要集中在400～700.
이탈지순용단백위저물진행매수해반응,획득료옥미원활성태조분,경포취당응효화리자교환등방법대산물진일보분리순화. 이α-생육분위양성대조,연구료활성태급기분리순화조분대2,2-이분기-1-고기분정(DPPH)자유기적청제활성급기농도적관계. 결과표명: 활성태급기분리순화조분균구유청제DPPH자유기적활성;경CM-섬유소약양리자교환층석분리소득조분2대DPPH자유기적청제활성최강,차청제효과여기농도급작용시간정정상관,분자량주요집중재400～700.
Defatted zein was hydrolyzed by proteolysis with protease. The hydrolysate was refined and freeze-dried into powder. The resulting hydrolysate was further purified by means of gel filtration,ion exchange chromatography and HPLC. With α-tocopherol as the positive control,the antioxidant abilities of corn peptide and its separated fractions were examined for their effects of scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical,dose-dependent manner was included. The data reveal that corn peptide and its separated fractions exhibited a potent effect of scavenging DPPH. The most active fraction from the active peptide was fraction 2 separated by CM cation exchange chromatography. Its scavenging effect is positively related to its concentration and the reaction time. The molecular-mass distributions of the fraction 2 are mostly in a range from 400～700.
