基础医学与临床
基礎醫學與臨床
기출의학여림상
BASIC MEDICAL SCIENCES AND CLINICS
2010年
3期
272-274
,共3页
肝癌%P57~(kip2)%遗传不稳定性%杂合性缺失%微卫星不稳定性
肝癌%P57~(kip2)%遺傳不穩定性%雜閤性缺失%微衛星不穩定性
간암%P57~(kip2)%유전불은정성%잡합성결실%미위성불은정성
HCC%P57~(kip2)%genetic instability%LOH%MSI
目的 探讨肝细胞癌(HCC)P57~(kip2) mRNA表达与遗传不稳定性之间的相互关系,以探索P57~(kip2)基因失表达的机制.方法 用原位分子杂交技术检测肝癌组织中尸57~(kip2)mRNA表达,用PCR-聚丙烯酰胺凝胶电泳一银染法检测LOH和MSI.结果 在正常肝组织未见P57~(kip2) mRNA表达,癌周肝硬化组与肝癌组阳性表达率均为26.7%(8/30).3个位点均未发生LOH;在2个微卫星位点发生MSI,MSI总阳性率为16.7%.D11Si760位点发生MSI与P57~(kip2) mRNA表达有关联性(P＜0.05).结论 P57~(kip2) mRNA表达异常提示其可能在HCC发生过程中起重要作用.MSI可能是肝癌发生过程中P57~(kip2) mRNA表达异常的原因之一.
目的 探討肝細胞癌(HCC)P57~(kip2) mRNA錶達與遺傳不穩定性之間的相互關繫,以探索P57~(kip2)基因失錶達的機製.方法 用原位分子雜交技術檢測肝癌組織中尸57~(kip2)mRNA錶達,用PCR-聚丙烯酰胺凝膠電泳一銀染法檢測LOH和MSI.結果 在正常肝組織未見P57~(kip2) mRNA錶達,癌週肝硬化組與肝癌組暘性錶達率均為26.7%(8/30).3箇位點均未髮生LOH;在2箇微衛星位點髮生MSI,MSI總暘性率為16.7%.D11Si760位點髮生MSI與P57~(kip2) mRNA錶達有關聯性(P＜0.05).結論 P57~(kip2) mRNA錶達異常提示其可能在HCC髮生過程中起重要作用.MSI可能是肝癌髮生過程中P57~(kip2) mRNA錶達異常的原因之一.
목적 탐토간세포암(HCC)P57~(kip2) mRNA표체여유전불은정성지간적상호관계,이탐색P57~(kip2)기인실표체적궤제.방법 용원위분자잡교기술검측간암조직중시57~(kip2)mRNA표체,용PCR-취병희선알응효전영일은염법검측LOH화MSI.결과 재정상간조직미견P57~(kip2) mRNA표체,암주간경화조여간암조양성표체솔균위26.7%(8/30).3개위점균미발생LOH;재2개미위성위점발생MSI,MSI총양성솔위16.7%.D11Si760위점발생MSI여P57~(kip2) mRNA표체유관련성(P＜0.05).결론 P57~(kip2) mRNA표체이상제시기가능재HCC발생과정중기중요작용.MSI가능시간암발생과정중P57~(kip2) mRNA표체이상적원인지일.
Objective To study the expressions of P57~(kip2) mRNA and genetic instability of P57~(kip2) in human hepatocellular carcinoma (HCC).Methods In situ hybridization(ISH) was used to detect the expression of P57~(kip2) mRNA in HCC.MSI and LOH were detected by PCR-polyacrylamide gel electmphoresis-silver staining method.Results There was no expression of P57~(kip2) mRNA found in normal liver tissue.The expression rate of P57~(kip2) mRNA in both pericancerous cirrhosis and hepatocellular carcinoma was 26.7%(8/30).LOH was not identified in 30 cases on three microsatellite loeies;there were 2 microsatellite loeies showing MSI,the total rate of MSI was 16.7%.There was correlation found between the MSI of D11S1760 locies and the expression of P57~(kip2) mRNA(P＜0.05).Conclusion The disorder expression of P57~(kip2) mRNA indicated that P57~(kip2) might be involved in hepatocarcinogenesis and prognosis.MSI may be one of the reasons that explains the disorder expression of P57~(kip2) mRNA in hepatocarcinogenesis and prognosis.
