CAJ | 학술논문

背景与目的:有研究提示某些化疗药物在小剂量应用时具有一定的抗血管生成作用,因此本研究旨在探讨小剂量羟基喜树碱(hydroxycamptothecin,HCPT)对小鼠H22细胞移植瘤的生长抑制作用及对血管生成的抑制作用.方法:建立小鼠皮下肝癌H22模型:以环磷酰胺(cyclophosphamide,CTX)为阳性对照,0.9%氯化钠溶液为阴性对照,HCPT(0.2和0.4 mg/kg)连续腹腔给药10 d,观察用药后移植肿瘤生长情况,计算抑瘤率:免疫组织化学法检测肿瘤组织中血管内皮生长因子(vascUlar endothelial growth factor,VEGF)的表达及微血管密度(microvessel density,MVD).结果:HCPT抑瘤作用明显,0.2和0.4mg/kg治疗组的抑瘤率分别为23.53%和43.25%,与0.9%氯化钠溶液对照组相比,差异有统计学意义(P<0.05);免疫组织化学检测结果显示各治疗组H22肿瘤组织中的VEGF和MVD的表达也明显低于阴性对照组(P<0.05).结论:小剂量HCPT对小鼠H22移植瘤具有一定的生长抑制作用;同时可明显抑制肿瘤组织中VEGF的表达水平和MVD,推测其抗肿瘤作用可能与对血管生成抑制作用有关.
배경여목적:유연구제시모사화료약물재소제량응용시구유일정적항혈관생성작용,인차본연구지재탐토소제량간기희수감(hydroxycamptothecin,HCPT)대소서H22세포이식류적생장억제작용급대혈관생성적억제작용.방법:건립소서피하간암H22모형:이배린선알(cyclophosphamide,CTX)위양성대조,0.9%록화납용액위음성대조,HCPT(0.2화0.4 mg/kg)련속복강급약10 d,관찰용약후이식종류생장정황,계산억류솔:면역조직화학법검측종류조직중혈관내피생장인자(vascUlar endothelial growth factor,VEGF)적표체급미혈관밀도(microvessel density,MVD).결과:HCPT억류작용명현,0.2화0.4mg/kg치료조적억류솔분별위23.53%화43.25%,여0.9%록화납용액대조조상비,차이유통계학의의(P<0.05);면역조직화학검측결과현시각치료조H22종류조직중적VEGF화MVD적표체야명현저우음성대조조(P<0.05).결론:소제량HCPT대소서H22이식류구유일정적생장억제작용;동시가명현억제종류조직중VEGF적표체수평화MVD,추측기항종류작용가능여대혈관생성억제작용유관.
Background and purpose: It has been reported that some low dose chemotherapy drugs have antiangiogenetic effects. The purpose of this study was to investigate the effect of inhibiting angiogenesis by low dose hydroxycamptothecin on H22 hepatoma transplantation tumor mouse models. Methods: H22 transplantation tumor mouse models were established, CTX was administrated in abdominal cavities as positive control group. 0.9%NaC1 solution was administrated as negative control group. Intra abdominal cyclophosphamide and hydroxycamptothecin (0.2 and 0.4 mg/kg) were injected for 10 days continuously. The growth of tumor were observed and measured. The tumor inhibitory rates were tested in animal tumor model with experimental treatment. The expression of VEGF and CD34 were measured by means of immunohistochemistry. Results: Hydroxycamptothecin had effect on tumor growth. Tumor weight inhibitory rates of hydroxycamptothecin with 0.2 and 0.4 mg/kg were 23.53% and 43.25% respectively. The difference was significant when compared with the negative control group (P<0.05). The expression of VEGF and MVD can be suppressed significantly than negative control group in vivo (P<0.05). Conclusion:Hydroxycamptothecin have inhibitory effect on tumor growth and the expression of VEGF and MVD with H22 hepatoma transplantation tumor mouse models in low dose. The mechanism possibly involved inhibiting the angiogenesis.