CAJ | 학술논문

目的建立测定头孢克肟有关物质的RP-HPLC方法.方法采用Hypersil BDS C_(18)(4.6mm×250mm,10μm)色谱柱,流动相为0.1mol/L醋酸铵缓冲液(用氨试液调pH为7.0)-乙腈(96:4),流速1.0mL/min,检测波长254nm,柱温35℃.结果头孢克肟在0.10-49.33μg/mL浓度范围内,峰面积与浓度呈良好线性关系(r=1.0000);最低检测限为0.18ng;各有关物质与主成份均达到基线分离.结论本法简便、专属,可用于头孢克肟有关物质的测定.
목적 건립측정두포극우유관물질적RP-HPLC방법.방법 채용Hypersil BDS C_(18)(4.6mm×250mm,10μm)색보주,류동상위0.1mol/L작산안완충액(용안시액조pH위7.0)-을정(96:4),류속1.0mL/min,검측파장254nm,주온35℃.결과 두포극우재0.10-49.33μg/mL농도범위내,봉면적여농도정량호선성관계(r=1.0000);최저검측한위0.18ng;각유관물질여주성빈균체도기선분리.결론 본법간편、전속,가용우두포극우유관물질적측정.
Objective To establish a method for the determination of related substances in cefixime by RPHPLC. Methods The chromatographic separation of the related substances was performed on a Hypersil BDS C_(18) column(4.6mm×250mm, 10μm)with a mixture of 0.1 mol/L ammonium acetate (adjusted with ammonia solution to pH of 7.0) and acetonitrile (96 : 4) as mobile phase. The flow rate was 1.0mL/min. The detection wavelength was 254nm. The column temperature was 35℃. Results The calibration curve was linear within the range of 0.10-49.33μg/mL for cefixime (r= 1.0000). The detection limit of cefixime was 0.18ng. The cefixime could be separated effectively from its degradation product with the method. Conclusion This method is simple and selective for the determination of related substances in Cefixime.