CAJ | 학술논문

目的：为进一步提高产乳糖酶酵母Kluyveromyces fragilis(K.fragilis)株的酶产量，对该菌株的培养产酶发酵条件进行优化试验。方法：采用增殖和诱导二步培养法培养酵母K.fragilis株，并采用单因素试验方法分析碳源、氮源对酵母诱导产酶的影响。结果：确定了K.fragilis酵母株诱导培养的较佳配方，当乳糖浓度为120g/L，醋酸铵浓度为5g/L，pH为6.7时，单位酶产量达122.4IU/ml培养液。结论：该菌株生长产酶情况良好，具有较高的应用价值。
목적：위진일보제고산유당매효모Kluyveromyces fragilis(K.fragilis)주적매산량，대해균주적배양산매발효조건진행우화시험。방법：채용증식화유도이보배양법배양효모K.fragilis주，병채용단인소시험방법분석탄원、담원대효모유도산매적영향。결과：학정료K.fragilis효모주유도배양적교가배방，당유당농도위120g/L，작산안농도위5g/L，pH위6.7시，단위매산량체122.4IU/ml배양액。결론：해균주생장산매정황량호，구유교고적응용개치。
Objective: To study the optimal condition on lactase production by yeast Kluyveromyces fragilis(K.fragilis) to increase the enzyme productivity.Methods: With separated proliferation and induction culture,the effe cts of carbon source and nitrogen source on yeast proliferation and lactase indu ction were studied by the method of monofactorial analysis.Results: The optimum medium for the productivity of lactase by K.f ragilis was determined.Maximal enzyme activity (122.4 IU/ml) was reached when the concentration of lactose was 12%,ammonium acetate was 0.5% and pH 6.7.Conclusion: The results of this experiment demonstrate that this str ain will be good for industrial production.