CAJ | 학술논문

背景:研究已经证实海藻糖对低温保存中的胸骨具有保护作用,但作用途径尚不清楚.目的:观察海藻糖对低温保存胸骨bcl-2和bax基因表达的影响.方法:新鲜配置4组保存液:低钾右旋糖酐组、低钾右旋糖酐+二甲基亚砜组、低钾右旋糖酐+海藻糖组、低钾右旋糖酐+二甲基亚砜组+海藻糖组.切取SD大鼠胸骨后立即分别放入含上述4 种溶液的冻存管中低温保存.抽取新鲜大鼠胸骨组织和低温保存120 d的4组标本,采用RT-PCR方法检测不同保存液保存的胸骨及新鲜胸骨bcl-2和bax基因mRNA表达量.结果与结论:低钾右旋糖酐+海藻糖组bcl-2表达量高于低钾右旋糖酐组、低钾右旋糖酐+二甲基亚砜组(P < 0.01),但bax表达量低于低钾右旋糖酐组、低钾右旋糖酐+二甲基亚砜组(P < 0.01);低钾右旋糖酐+二甲基亚砜组+海藻糖组bcl-2表达量最高,bax表达量最低,基本接近新鲜骨组织(P > 0.05).结果提示海藻糖可能通过增强bcl-2基因、抑制bax基因的表达保护低温保存中的胸骨细胞活性,其与二甲基亚砜合用保护作用更好.
배경:연구이경증실해조당대저온보존중적흉골구유보호작용,단작용도경상불청초.목적:관찰해조당대저온보존흉골bcl-2화bax기인표체적영향.방법:신선배치4조보존액:저갑우선당항조、저갑우선당항+이갑기아풍조、저갑우선당항+해조당조、저갑우선당항+이갑기아풍조+해조당조.절취SD대서흉골후립즉분별방입함상술4 충용액적동존관중저온보존.추취신선대서흉골조직화저온보존120 d적4조표본,채용RT-PCR방법검측불동보존액보존적흉골급신선흉골bcl-2화bax기인mRNA표체량.결과여결론:저갑우선당항+해조당조bcl-2표체량고우저갑우선당항조、저갑우선당항+이갑기아풍조(P < 0.01),단bax표체량저우저갑우선당항조、저갑우선당항+이갑기아풍조(P < 0.01);저갑우선당항+이갑기아풍조+해조당조bcl-2표체량최고,bax표체량최저,기본접근신선골조직(P > 0.05).결과제시해조당가능통과증강bcl-2기인、억제bax기인적표체보호저온보존중적흉골세포활성,기여이갑기아풍합용보호작용경호.
BACKGROUND: Studies have demonstrated that trehalose possesses protective effects on cyropreserved sternum. But the mechanism of action remains poorly understood. OBJECTIVE: To investigate the effects of trehalose on bcl-2 and bax mRNA expression in cryopreserved sternum. METHODS: Four groups of freshly prepared solution were used: low-potassium dextran (LPD), LPD + dimethyl sulfoxide (DMSO), LPD + trehalose, LPD + DMSO + trehalose. Rat sternum was cut and then immediately cryopreserved in the tubes containing each group of solution. Fresh rat sternum tissue and 4 groups of samples cryopreserved for 120 days were taken and bcl-2 and bax mRNA expression in fresh and cryopreserved sternum was detected using reverse transcription-polymerase chain reaction.RESULTS AND CONCLUSION: bcl-2 mRNA expression in the LPD + trehalose group was significantly higher, but bax mRNA expression was significantly lower, than in the LPD, LPD + DMSO groups (both P < 0.01). LPD + DMSO + trehalose group showed highest bcl-2 mRNA expression and lowest bax mRNA expression, which were basically similar to fresh bone tissue (P > 0.05). These findings indicate that trehalose may protect cell activity in cryopreserved sternum by enhancing bcl-2 mRNA expression and inhibiting bax mRNA expression, and trehalose together with DMSO shows better protective effects.