中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2012年
11期
2028-2032
,共5页
微透析技术%高效液相色谱法%玻璃体%万古霉素%药代动力学
微透析技術%高效液相色譜法%玻璃體%萬古黴素%藥代動力學
미투석기술%고효액상색보법%파리체%만고매소%약대동역학
背景:万古霉素玻璃体内注射后的药代动力学资料较少.目的:观察万古霉素玻璃体腔内注射后在清醒兔眼玻璃体内的浓度.方法:将微透析探针植入清醒正常兔眼和细菌性眼内炎兔眼玻璃体内24 h 后,向玻璃体内注射10 g/L 万古霉素0.1 mL,利用微透析采样技术联合高效液相色谱法连续检测万古霉素注射后0.5,1,2,4,6,12,24,48,72,84 h,兔眼玻璃体内的药物浓度.结果与结论:万古霉素在正常兔眼玻璃体内的代谢呈开放式二室模型,玻璃体内的峰值浓度为695.92 mg/L,半衰期51.66 h;在细菌性眼内炎兔眼玻璃体内的代谢呈一室模型,万古霉素的峰值浓度为713.35 mg/L,半衰期为11.91 h.所有动物给药84 h,玻璃体内万古霉素的浓度均高于最小抑菌浓度.实验在动物清醒状态下实时、连续、动态采样,检测结果准确,能满足万古霉素药动学分析的需要.
揹景:萬古黴素玻璃體內註射後的藥代動力學資料較少.目的:觀察萬古黴素玻璃體腔內註射後在清醒兔眼玻璃體內的濃度.方法:將微透析探針植入清醒正常兔眼和細菌性眼內炎兔眼玻璃體內24 h 後,嚮玻璃體內註射10 g/L 萬古黴素0.1 mL,利用微透析採樣技術聯閤高效液相色譜法連續檢測萬古黴素註射後0.5,1,2,4,6,12,24,48,72,84 h,兔眼玻璃體內的藥物濃度.結果與結論:萬古黴素在正常兔眼玻璃體內的代謝呈開放式二室模型,玻璃體內的峰值濃度為695.92 mg/L,半衰期51.66 h;在細菌性眼內炎兔眼玻璃體內的代謝呈一室模型,萬古黴素的峰值濃度為713.35 mg/L,半衰期為11.91 h.所有動物給藥84 h,玻璃體內萬古黴素的濃度均高于最小抑菌濃度.實驗在動物清醒狀態下實時、連續、動態採樣,檢測結果準確,能滿足萬古黴素藥動學分析的需要.
배경:만고매소파리체내주사후적약대동역학자료교소.목적:관찰만고매소파리체강내주사후재청성토안파리체내적농도.방법:장미투석탐침식입청성정상토안화세균성안내염토안파리체내24 h 후,향파리체내주사10 g/L 만고매소0.1 mL,이용미투석채양기술연합고효액상색보법련속검측만고매소주사후0.5,1,2,4,6,12,24,48,72,84 h,토안파리체내적약물농도.결과여결론:만고매소재정상토안파리체내적대사정개방식이실모형,파리체내적봉치농도위695.92 mg/L,반쇠기51.66 h;재세균성안내염토안파리체내적대사정일실모형,만고매소적봉치농도위713.35 mg/L,반쇠기위11.91 h.소유동물급약84 h,파리체내만고매소적농도균고우최소억균농도.실험재동물청성상태하실시、련속、동태채양,검측결과준학,능만족만고매소약동학분석적수요.
BACKGROUND: There are currently few studies regarding the pharmacokinetics of vancomycin via intravitreous injection.OBJECTIVE: To determine the concentration of vancomycin injected into the vitreous chamber of conscious rabbits.METHODS: A microdialysis probe was implanted into vitreous chamber of normal rabbit eyes and rabbit eyes infected withbacterial endophthalmitis for 24 hours, and 10 g/L vancomycin 0.1 mL was administered intravitreally. The drug concentration inthe vitreous chamber of rabbit eyes was determined at 0.5, 1, 2, 4, 6, 12, 24, 48, 72 and 84 hours after injection, through themicrodialysis and high performance liquid chromatogram-ultraviolet detection.RESULTS AND CONCLUSION: The metabolism of vancomycin showed an open two-compartment model in normal rabbit eyes.Its half-life was 51.66 hours and the peak concentration was 695.92 mg/L. The metabolism of vancomycin in the infected vitreouschamber showed a one-compartment model. Its half-life was 11.91 hours and the peak concentration was 713.35 mg/L. All rabbitswere injected with drugs for 84 hours and the intravitreous concentration of vancomycin was higher than minimal inhibitoryconcentration. The experimental findings indicate that microdialysis coupled to high performance liquid chromatography is apowerful tool to investigate the ocular pharmacokinetics of vancomycin, and the samples are harvested in a real-time, continuousand dynamic fashion when the experimental animals are conscious.
