国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2010年
6期
502-503,534
,共3页
心肺转流术%红细胞%细胞内钙离子浓度
心肺轉流術%紅細胞%細胞內鈣離子濃度
심폐전류술%홍세포%세포내개리자농도
Cardiopulmonary bypass%Erythrocyte%Intracell calcium content
目的 通过比较先天性心脏病患儿体外循环(cardiepulmonary bypass,CPB)过程中红细胞内外钙离子浓度的变化,探讨CPB对红细胞形态和功能的影响.方法 分别于CPB前、中、后抽取26例先天性心脏病患儿的中心静脉血检测血浆游离钙离子浓度,同时采用Fluo-3-AM测定红细胞内的钙离子浓度.结果 CPB中血浆钙离子浓度由CPB前的(1.24±0.06)mmol/L降低至(0.99±0.05)mmol/L,CPB后钙离子浓度又恢复至(1.28±0.08)mmol/L;CPB过程中红细胞内钙离子浓度则维持了相对稳定[CPB前(138±72)nmol/L;CPB中(145±70)nmol/L;CPB后(115±83)nmol/L].结论 CPB期间红细胞内钙离子浓度无显著变化.
目的 通過比較先天性心髒病患兒體外循環(cardiepulmonary bypass,CPB)過程中紅細胞內外鈣離子濃度的變化,探討CPB對紅細胞形態和功能的影響.方法 分彆于CPB前、中、後抽取26例先天性心髒病患兒的中心靜脈血檢測血漿遊離鈣離子濃度,同時採用Fluo-3-AM測定紅細胞內的鈣離子濃度.結果 CPB中血漿鈣離子濃度由CPB前的(1.24±0.06)mmol/L降低至(0.99±0.05)mmol/L,CPB後鈣離子濃度又恢複至(1.28±0.08)mmol/L;CPB過程中紅細胞內鈣離子濃度則維持瞭相對穩定[CPB前(138±72)nmol/L;CPB中(145±70)nmol/L;CPB後(115±83)nmol/L].結論 CPB期間紅細胞內鈣離子濃度無顯著變化.
목적 통과비교선천성심장병환인체외순배(cardiepulmonary bypass,CPB)과정중홍세포내외개리자농도적변화,탐토CPB대홍세포형태화공능적영향.방법 분별우CPB전、중、후추취26례선천성심장병환인적중심정맥혈검측혈장유리개리자농도,동시채용Fluo-3-AM측정홍세포내적개리자농도.결과 CPB중혈장개리자농도유CPB전적(1.24±0.06)mmol/L강저지(0.99±0.05)mmol/L,CPB후개리자농도우회복지(1.28±0.08)mmol/L;CPB과정중홍세포내개리자농도칙유지료상대은정[CPB전(138±72)nmol/L;CPB중(145±70)nmol/L;CPB후(115±83)nmol/L].결론 CPB기간홍세포내개리자농도무현저변화.
Objective To study the changes of intra-erythrocyte calcium concentration during cardiopulrnonary bypass( CPB ).Methods 26 pediatric patients undergoing surgical repair of ventricular or atrial sepal defect under CPB were enrolled in this prospective study. Heparinized venous blood samples were taken from the central vein before CPB, at the beginning of CPB and after CPB, respectively. The concentrations of intra-erythrocyte [ Ca2+] were measured by fluorescent probes (Fluo-3-AM), while plasma [Ca2+] were determined by blood gas analyzer. Results Before CPB and after CPB, the concentrations of plasma[ Ca2+]were ( 1.24±0.06) mmol/L and (1.28±0.08) mmol/L, respectively. It remarkably decreased at the beginning of CPB (0.99±0.05) mmol/L. There were no significant differences in the levels of intra-erythrocyte[ Ca2+ ] concentration among the three time points[ (138±72) nmol/L vs (145±70) nmol/L vs (115±83) nmol/L]. Conclusion CPB doesn't change the intra-erythrocyte calcium concentrations.
