中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2008年
2期
125-127
,共3页
王长华%李艳芝%张志新%黄家红%高国花%谢小英%刘丽娜
王長華%李豔芝%張誌新%黃傢紅%高國花%謝小英%劉麗娜
왕장화%리염지%장지신%황가홍%고국화%사소영%류려나
高胆红素血症%光疗%氧化应激%新生儿
高膽紅素血癥%光療%氧化應激%新生兒
고담홍소혈증%광료%양화응격%신생인
Hyperbilirubinemia%Phototheraphy%Oxidative stress%Neonates
目的 探讨光照疗法(光疗)对高胆红素血症新生儿氧化应激反应的诱导效应.方法 将60例非溶血性高胆红素血症新生儿随机分为两组:光疗1组30例,入院后持续光疗24 h;光疗2组30例,入院24 h内不予光疗.用比色法测定两组患儿的血清丙二醛(MDA)浓度,血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)性.结果 光疗1组患儿光疗24 h后血清MDA浓度为(18.59±3.11)nmol/ml,明显高于光疗前[(16.13±3.12)nmol/ml],差异有非常显著性(P＜0.01);血清SOD、CAT、GSH-Px活性分别为(63.92±12.40)U/ml、(1.60±0.44)U/ml与(74.33±31.48)酶活力单位,明显低于光疗前[(75.80±13.78)U/ml、(1.87±0.51)U/ml与(90.52±29.88)酶活力单位],差异有非常显著性(P均＜0.01).光疗2组患儿一般治疗24 h后血清MDA浓度,SOD、CAT、GSH-Px活性与治疗前相比,差异无显著性(P均＞0.05).结论 光疗可诱导新生儿氧化应激反应.
目的 探討光照療法(光療)對高膽紅素血癥新生兒氧化應激反應的誘導效應.方法 將60例非溶血性高膽紅素血癥新生兒隨機分為兩組:光療1組30例,入院後持續光療24 h;光療2組30例,入院24 h內不予光療.用比色法測定兩組患兒的血清丙二醛(MDA)濃度,血清超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、穀胱甘肽過氧化物酶(GSH-Px)性.結果 光療1組患兒光療24 h後血清MDA濃度為(18.59±3.11)nmol/ml,明顯高于光療前[(16.13±3.12)nmol/ml],差異有非常顯著性(P＜0.01);血清SOD、CAT、GSH-Px活性分彆為(63.92±12.40)U/ml、(1.60±0.44)U/ml與(74.33±31.48)酶活力單位,明顯低于光療前[(75.80±13.78)U/ml、(1.87±0.51)U/ml與(90.52±29.88)酶活力單位],差異有非常顯著性(P均＜0.01).光療2組患兒一般治療24 h後血清MDA濃度,SOD、CAT、GSH-Px活性與治療前相比,差異無顯著性(P均＞0.05).結論 光療可誘導新生兒氧化應激反應.
목적 탐토광조요법(광료)대고담홍소혈증신생인양화응격반응적유도효응.방법 장60례비용혈성고담홍소혈증신생인수궤분위량조:광료1조30례,입원후지속광료24 h;광료2조30례,입원24 h내불여광료.용비색법측정량조환인적혈청병이철(MDA)농도,혈청초양화물기화매(SOD)、과양화경매(CAT)、곡광감태과양화물매(GSH-Px)성.결과 광료1조환인광료24 h후혈청MDA농도위(18.59±3.11)nmol/ml,명현고우광료전[(16.13±3.12)nmol/ml],차이유비상현저성(P＜0.01);혈청SOD、CAT、GSH-Px활성분별위(63.92±12.40)U/ml、(1.60±0.44)U/ml여(74.33±31.48)매활력단위,명현저우광료전[(75.80±13.78)U/ml、(1.87±0.51)U/ml여(90.52±29.88)매활력단위],차이유비상현저성(P균＜0.01).광료2조환인일반치료24 h후혈청MDA농도,SOD、CAT、GSH-Px활성여치료전상비,차이무현저성(P균＞0.05).결론 광료가유도신생인양화응격반응.
Objective To explore whether phototherapy induces an oxidative stress in neonates with hyperbilirubinemia.Methods Sixty neonates with hyperbilirubinemia were randomly divided into two groups,group one(30 cases)and group two(30 cases).Patients of group one were given with 24 hours of continuous phototherapy after admission.Patients Of group two were not given with phototherapy within 24 hours and only given with routine treatment.And then,we measured the level of malondialdehyde(MDA)and serum activitles of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)by chromatometrv.Results In group one,the serum level of MDA after 24 hours of continuous phototheraPY was significantly higher than that before phototherapy(18.59±3.11 vs 16.13±3.12)nmol/ml(P＜0.01),the serum activities of SOD,CAT and GSH-Px were significantly lower than those before phototherapy[(63.92±12.40 vs 75.80±13.78)U/ml,(1.60±0.44 vs 1.87±0.51)U/ml,(74.33±31.48 vs 90.52±29.88)unit of activity](p＜0.01).In group two,the serum level of MDA and the activities of SOD,CAT and GSH-Px were not different between before and after 24 hours of routine treatment(P＞0.05).Conclusion Phototherapy induces an oxidative stress in neonates.
