CAJ | 학술논문

目的探讨线粒体解耦联蛋白2 (UcP-2)基因启动子-866G＞A变异与2型糖尿病患者合并缺血性脑卒中(IS)发生风险的相关性.方法对844例2型糖尿病患者(伴IS组404例,不伴IS组440例)进行为期4年的前瞻性队列研究,提取所有受试者的基因组全血DNA,用TaqMan MGB探针对UCP-2基因启动子-866G＞A进行基因分型,分析不同基因型与2型糖尿病合并IS发生风险的关联性.结果无论在基线时还是在4年随访过程中,携带A等位基因的2型糖尿病女性患者IS发生风险明显高于GG野生型(P＜0.05),但男性患者3种基因型之间差异无统计学意义.结论 UCP-2基因启动子-866G＞A变异增加中国女性2型糖尿病并发IS的风险.
목적 탐토선립체해우련단백2 (UcP-2)기인계동자-866G＞A변이여2형당뇨병환자합병결혈성뇌졸중(IS)발생풍험적상관성.방법 대844례2형당뇨병환자(반IS조404례,불반IS조440례)진행위기4년적전첨성대렬연구,제취소유수시자적기인조전혈DNA,용TaqMan MGB탐침대UCP-2기인계동자-866G＞A진행기인분형,분석불동기인형여2형당뇨병합병IS발생풍험적관련성.결과 무론재기선시환시재4년수방과정중,휴대A등위기인적2형당뇨병녀성환자IS발생풍험명현고우GG야생형(P＜0.05),단남성환자3충기인형지간차이무통계학의의.결론 UCP-2기인계동자-866G＞A변이증가중국녀성2형당뇨병병발IS적풍험.
Objective To investigate the association of uncoupling protein 2 ( UCP-2 ) gene promoter -866G＞A polymorphism and ischemic stroke in diabetic patients.Methods A total of 844 type 2 diabetic patients including 404 cases with ischemic stroke and 440 cases without ischemic stroke were selected for the 4 year prospective study,Genomic DNA was extracted from the whole blood samples of subjects,UCP-2 gene promoter -866G ＞ A polymorphism was detected by TaqMan MGB probe method,and then the genotype and allele gene frequencies were compared.Results The risk of ischemic stroke in type 2 diabetic female patients with AA+GA genotypes of UCP-2 was higher than that with GG genotype (P＜0.05),but there was no difference among male patients with three genotypes.Conclusions UCP-2 gene promoter -866G ＞ A polymorphism increases the risk of ischemic stroke in Chinese diabetic women.