中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
8期
611-613
,共3页
焦路阳%郭庆合%宋志善%鲁广建
焦路暘%郭慶閤%宋誌善%魯廣建
초로양%곽경합%송지선%로엄건
百草枯%急性肾损伤%粒细胞明胶酶相关脂质运载蛋白
百草枯%急性腎損傷%粒細胞明膠酶相關脂質運載蛋白
백초고%급성신손상%립세포명효매상관지질운재단백
Paraquat%Acute kidney injury%Urinary neutrophil gelatinase-associated lipocalin (uNGAL)
目的 探讨尿中性粒细胞明胶酶相关脂质运载蛋白(uNGAL)对百草枯中毒患者急性肾损伤(AKI)的早期诊断价值.方法 以我院急诊科收治的85例患者为观察对象,收集百草枯中毒入院后即刻(15 min内)及2、4、6、8、10、12、18、24、36、48及72 h,5和7 d时的血、尿液标本各5ml,用酶联免疫吸附(ELISA)法检测尿中uNGAL水平.同时用肌氨酸氧化酶法测定血清肌酐(SCr)水平.结果 百草枯中毒患者中发生AKI 62例,发生率为72.94%(62/85).AKI患者18、24、36、48、72 h及5、7 d各时间点的SCr水平明显上升,与基线值和对照组比较,差异有统计学意义(P＜0.01).AKI组24、36、48、72h及5、7 d各时间点的SCr水平与非AKI组比较,差异有统计学意义(P＜0.01).百草枯中毒AKI组2 h尿中uNGAL即明显高于基线值,达(96.21 ±45.32)μg/L.4、6、8、10、12、18、36、36、48、72 h及5、7 d各时间点百草枯中毒AKI组患者尿中uNGAL水平呈逐渐升高的趋势,与对照组和基线值比较,差异均有统计学意义(P＜0.01).与对照组和基线值比较,12、18、24、36、48、72h及5、7d各时间点非AKI组尿中uNGAL水平明显升高,差异有统计学意义(P＜0.05).各时间点百草枯中毒AKI组尿中uNGAL水平均明显高于非AKI组,差异有统计学意义(P＜0.01).结论 百草枯中毒后2 h尿中uNGAL水平明显升高,其诊断AKI的时间早于SCr.尿中uNGAL可作为百草枯中毒后AKI的早期诊断标志物.
目的 探討尿中性粒細胞明膠酶相關脂質運載蛋白(uNGAL)對百草枯中毒患者急性腎損傷(AKI)的早期診斷價值.方法 以我院急診科收治的85例患者為觀察對象,收集百草枯中毒入院後即刻(15 min內)及2、4、6、8、10、12、18、24、36、48及72 h,5和7 d時的血、尿液標本各5ml,用酶聯免疫吸附(ELISA)法檢測尿中uNGAL水平.同時用肌氨痠氧化酶法測定血清肌酐(SCr)水平.結果 百草枯中毒患者中髮生AKI 62例,髮生率為72.94%(62/85).AKI患者18、24、36、48、72 h及5、7 d各時間點的SCr水平明顯上升,與基線值和對照組比較,差異有統計學意義(P＜0.01).AKI組24、36、48、72h及5、7 d各時間點的SCr水平與非AKI組比較,差異有統計學意義(P＜0.01).百草枯中毒AKI組2 h尿中uNGAL即明顯高于基線值,達(96.21 ±45.32)μg/L.4、6、8、10、12、18、36、36、48、72 h及5、7 d各時間點百草枯中毒AKI組患者尿中uNGAL水平呈逐漸升高的趨勢,與對照組和基線值比較,差異均有統計學意義(P＜0.01).與對照組和基線值比較,12、18、24、36、48、72h及5、7d各時間點非AKI組尿中uNGAL水平明顯升高,差異有統計學意義(P＜0.05).各時間點百草枯中毒AKI組尿中uNGAL水平均明顯高于非AKI組,差異有統計學意義(P＜0.01).結論 百草枯中毒後2 h尿中uNGAL水平明顯升高,其診斷AKI的時間早于SCr.尿中uNGAL可作為百草枯中毒後AKI的早期診斷標誌物.
목적 탐토뇨중성립세포명효매상관지질운재단백(uNGAL)대백초고중독환자급성신손상(AKI)적조기진단개치.방법 이아원급진과수치적85례환자위관찰대상,수집백초고중독입원후즉각(15 min내)급2、4、6、8、10、12、18、24、36、48급72 h,5화7 d시적혈、뇨액표본각5ml,용매련면역흡부(ELISA)법검측뇨중uNGAL수평.동시용기안산양화매법측정혈청기항(SCr)수평.결과 백초고중독환자중발생AKI 62례,발생솔위72.94%(62/85).AKI환자18、24、36、48、72 h급5、7 d각시간점적SCr수평명현상승,여기선치화대조조비교,차이유통계학의의(P＜0.01).AKI조24、36、48、72h급5、7 d각시간점적SCr수평여비AKI조비교,차이유통계학의의(P＜0.01).백초고중독AKI조2 h뇨중uNGAL즉명현고우기선치,체(96.21 ±45.32)μg/L.4、6、8、10、12、18、36、36、48、72 h급5、7 d각시간점백초고중독AKI조환자뇨중uNGAL수평정축점승고적추세,여대조조화기선치비교,차이균유통계학의의(P＜0.01).여대조조화기선치비교,12、18、24、36、48、72h급5、7d각시간점비AKI조뇨중uNGAL수평명현승고,차이유통계학의의(P＜0.05).각시간점백초고중독AKI조뇨중uNGAL수평균명현고우비AKI조,차이유통계학의의(P＜0.01).결론 백초고중독후2 h뇨중uNGAL수평명현승고,기진단AKI적시간조우SCr.뇨중uNGAL가작위백초고중독후AKI적조기진단표지물.
Objective To explore the use of the urinary neutrophil gelatinase associated lipocalin (uNGAL) in the early diagnosis of paraquat poisoning patients with acute kidney injury (AKI).Methods Eighty five patients were from the emergency department in our hospital.Five ml blood and urine were collected from each patient at 15 min, 2, 4, 6, 8, 10, 12, 18, 24, 36, 48 and 72 h, 5 and 7d after admission.The uNGAL levels of urine were detected with ELISA test and the SCr levels were measured with creatine oxidase assay.Results Sixty two eases of paraquat intoxication suffered from AKI, the incidence was 72.94% (62/85).The SCr levels of 62 cases with AKI at 18, 24, 36, 48, 72 h and 5, 7 d after admission increased significantly, as compared with the baseline value and control group (P＜0.01).At 24, 36, 48, 72 h and 5, 7 d after admission,there was significant difference of the SCr levels between AKI group and non- AKI group (P＜0.01).At 2 h after admission, the uNGAL level of urine in paraquat intoxication AKI group was (96.21±45.32) μg/L which was significantly higher than the baseline value.At 10, 12, 18, 24, 36, 48, 72 h and 5, 7 d after admission, the uNGAL levels of urine in AKI group and non-AKI group obviously enhanced, as compared with the baseline value and control group (P＜0.01 or P＜0.05).At all time points, there was significant difference of the uNGAL level between AKI group and non-AKI group (P＜0.01).Conclusion The uNGAL level of urine in paraquat intoxication patients at 2 h after admission significantly enhanced, which is earlier than enhanced SCr.So the uNGAL level of urine may serve as early diagnostic biomarker for AKI induced by paraquat intoxication.
