中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2012年
7期
723-726
,共4页
郑伟文%黄昌明%谢建伟%郑朝辉%李平%王家镔%林建贤
鄭偉文%黃昌明%謝建偉%鄭朝輝%李平%王傢鑌%林建賢
정위문%황창명%사건위%정조휘%리평%왕가빈%림건현
胃肿瘤%微小RNA-183%埃兹蛋白%临床病理特征%预后
胃腫瘤%微小RNA-183%埃玆蛋白%臨床病理特徵%預後
위종류%미소RNA-183%애자단백%림상병리특정%예후
Stomach neoplasms%MicroRNA-183%Ezrin%Clinicopathologic features%Prognosis
目的 探讨微小RNA- 183(miR-183)和埃兹蛋白(Ezrin)在Ⅱ期胃癌中的表达情况及临床意义.方法 采用实时定量PCR检测72例Ⅱ期胃癌及癌旁组织中miR-183表达,免疫组织化学SP法检测相应癌组织中Ezrin表达,分析miR-183与Ⅱ期胃癌临床病理特征和预后的关系及与Ezrin表达的相关性.结果 miR-183在Ⅱ期胃癌组织中的表达低于癌旁组织(相对表达量中位值0.676比1.000,P＜0.05)；miR-183表达下调与肿瘤细胞分化程度(0.429比0.907,P＜0.05)和淋巴结转移相关(0.507比0.908,P＜0.05)；miR-183低表达组生存时间[(63.0±4.0)个月]低于高表达组[(75.2±3.8)个月,P＜0.05]；相关性分析显示,miR-183与Ezrin表达呈负相关(r=-0.272,P＜0.05).结论 miR-183在Ⅱ期胃癌中表达下调,与胃癌的分化、转移及预后相关,Ezrin可能为其调控蛋白之一.
目的 探討微小RNA- 183(miR-183)和埃玆蛋白(Ezrin)在Ⅱ期胃癌中的錶達情況及臨床意義.方法 採用實時定量PCR檢測72例Ⅱ期胃癌及癌徬組織中miR-183錶達,免疫組織化學SP法檢測相應癌組織中Ezrin錶達,分析miR-183與Ⅱ期胃癌臨床病理特徵和預後的關繫及與Ezrin錶達的相關性.結果 miR-183在Ⅱ期胃癌組織中的錶達低于癌徬組織(相對錶達量中位值0.676比1.000,P＜0.05)；miR-183錶達下調與腫瘤細胞分化程度(0.429比0.907,P＜0.05)和淋巴結轉移相關(0.507比0.908,P＜0.05)；miR-183低錶達組生存時間[(63.0±4.0)箇月]低于高錶達組[(75.2±3.8)箇月,P＜0.05]；相關性分析顯示,miR-183與Ezrin錶達呈負相關(r=-0.272,P＜0.05).結論 miR-183在Ⅱ期胃癌中錶達下調,與胃癌的分化、轉移及預後相關,Ezrin可能為其調控蛋白之一.
목적 탐토미소RNA- 183(miR-183)화애자단백(Ezrin)재Ⅱ기위암중적표체정황급림상의의.방법 채용실시정량PCR검측72례Ⅱ기위암급암방조직중miR-183표체,면역조직화학SP법검측상응암조직중Ezrin표체,분석miR-183여Ⅱ기위암림상병리특정화예후적관계급여Ezrin표체적상관성.결과 miR-183재Ⅱ기위암조직중적표체저우암방조직(상대표체량중위치0.676비1.000,P＜0.05)；miR-183표체하조여종류세포분화정도(0.429비0.907,P＜0.05)화림파결전이상관(0.507비0.908,P＜0.05)；miR-183저표체조생존시간[(63.0±4.0)개월]저우고표체조[(75.2±3.8)개월,P＜0.05]；상관성분석현시,miR-183여Ezrin표체정부상관(r=-0.272,P＜0.05).결론 miR-183재Ⅱ기위암중표체하조,여위암적분화、전이급예후상관,Ezrin가능위기조공단백지일.
Objective To investigate the expression of microRNA-183 (miR-183) and Erzrin protein in stage Ⅱ gastric cancer (GC).Methods Specimens of stage Ⅱ GC and paracancer tissues (5cm away from the tumor tissues) were collected from 72 patients.Real-time PCR was used to detect the miR-183 expression.Immunohistochemistry was used to examine the Ezrin protein expression in the tumor tissue.The associations of miR-183 expression with the clinicopathologic features of stage Ⅱ GC and Ezrin expression were analyzed.Results miR-183 expression was lower in stage Ⅱ gastric cancer tissues compared with the paracancer tissues samples (median relative expression,0.676 vs.1.000,P＜0.05).Low expression of miR-183 was significantly associated with histological differentiation (0.429 vs.0.907,P＜0.05),lymph node metastasis(0.507 vs.0.908,P＜0.05).The survival was shorter in patient with low expression of miR-183 (63.0±4.0) as compared to those with high expression of miR-183 (75.2±3.8) (P＜0.05).There was a negative correlation between the expression of miR-183 and Ezrin (r=-0.272,P＜0.05).Conclusions miR-183 is down-regulated in stage Ⅱ GC,and associated with the differentiation,metastasis,and prognosis.Ezrin is a potential regulatory protein of miR-183.
