中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
5期
885-887
,共3页
来森艳%王桂华%曹小年%李兆明%龚建平%胡俊波
來森豔%王桂華%曹小年%李兆明%龔建平%鬍俊波
래삼염%왕계화%조소년%리조명%공건평%호준파
胃肠道间质瘤%复发%伊马替尼耐药%上皮膜蛋白-1
胃腸道間質瘤%複髮%伊馬替尼耐藥%上皮膜蛋白-1
위장도간질류%복발%이마체니내약%상피막단백-1
Gastrointestinal stromal tumors%Recurrence%Imatinib-resistance%Epithelial membrane protein-1
目的 寻找胃肠道间质瘤(GIST)患者格列卫耐药的标记物,指导格列卫的临床用药.方法 采用免疫组织化学方法比较格列卫规范治疗后,耐药的患者耐药前后上皮膜蛋白-1( EMP-1)蛋白的表达水平.将GIST882细胞种植于裸鼠皮下形成移植瘤,裸鼠持续口服伊马替尼3周后传代,直至移植瘤耐药.比较耐药移植瘤与敏感移植瘤中EMP-1基因和蛋白表达水平的差异.结果 伊马替尼耐药后,患者及移植瘤肿瘤组织中EMP-1表达较耐药前显著增加,伊马替尼耐药移植瘤中EMP-1的RNA水平较敏感移植瘤增加约11倍.结论 EMP-1可以作为GIST耐药的标志物.
目的 尋找胃腸道間質瘤(GIST)患者格列衛耐藥的標記物,指導格列衛的臨床用藥.方法 採用免疫組織化學方法比較格列衛規範治療後,耐藥的患者耐藥前後上皮膜蛋白-1( EMP-1)蛋白的錶達水平.將GIST882細胞種植于裸鼠皮下形成移植瘤,裸鼠持續口服伊馬替尼3週後傳代,直至移植瘤耐藥.比較耐藥移植瘤與敏感移植瘤中EMP-1基因和蛋白錶達水平的差異.結果 伊馬替尼耐藥後,患者及移植瘤腫瘤組織中EMP-1錶達較耐藥前顯著增加,伊馬替尼耐藥移植瘤中EMP-1的RNA水平較敏感移植瘤增加約11倍.結論 EMP-1可以作為GIST耐藥的標誌物.
목적 심조위장도간질류(GIST)환자격렬위내약적표기물,지도격렬위적림상용약.방법 채용면역조직화학방법비교격렬위규범치료후,내약적환자내약전후상피막단백-1( EMP-1)단백적표체수평.장GIST882세포충식우라서피하형성이식류,라서지속구복이마체니3주후전대,직지이식류내약.비교내약이식류여민감이식류중EMP-1기인화단백표체수평적차이.결과 이마체니내약후,환자급이식류종류조직중EMP-1표체교내약전현저증가,이마체니내약이식류중EMP-1적RNA수평교민감이식류증가약11배.결론 EMP-1가이작위GIST내약적표지물.
Objective To find a biomarker of imatinib-resistant gastrointestinal stromal tumors ( GIST),and further to explore a solution to Imatinib-resistance.Methods To Compare the expression of epithelial membrane protein-1 ( EMP-1 ) between the Imatinib-sensitive GIST and the Imatinib-resistant GIST of the same patient by immunohistochemisty.A model of Imatinib-resistant nude mice xenograft was proposed by durative Imatinib treatment of the nude mice bearing subcutaneous GIST882 xenograft tumors.Then the expression of EMP-1 was compared in Imatinib-sensitive nude mice xenograft tumors and Imatinib-resistant xenograft tumors by immunohistochemisty and quantitative real-time polymerase chain reaction (qRT-PCR).Results EMP-1 was overexpressed in Imatinib-resistant GIST patient and nude mice xenograft tumors.The mRNA level in Imatinib-resistant nude mice xenograft tumors was 11-fold higher than that in Imatinib-sensitive nude mice xenograft tumor.
