中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2008年
2期
91-94
,共4页
孟维珊%李铭华%王环宇%张稽博%高煜%丁俊%付士红%翟友刚%陈哲%郭军巧%赵玉军%梁国栋
孟維珊%李銘華%王環宇%張稽博%高煜%丁俊%付士紅%翟友剛%陳哲%郭軍巧%趙玉軍%樑國棟
맹유산%리명화%왕배우%장계박%고욱%정준%부사홍%적우강%진철%곽군교%조옥군%량국동
脑炎病毒,日本%基因型%种系发生
腦炎病毒,日本%基因型%種繫髮生
뇌염병독,일본%기인형%충계발생
Encephalitis virus,Japanese%Genotype%Phylogeny
目的 了解2007年在辽宁省分离的乙型脑炎病毒基因型别及其病毒E基因分子特征.方法2006年8月在辽宁省东港市采集蚊虫标本,利用组织培养细胞进行病毒分离,对病毒分离物进行血清学和分子生物学鉴定.结果从采集的30批,共1500只三带喙库蚊标本中分离到2株病毒,命名为LNDG07-02、LNDG07-16,经鉴定均为基因1型乙脑病毒.病毒E基因区段核苷酸和氨基酸序列与乙脑减毒活疫苗株(SA14-14-2株)的同源性分别为87.8%～88.0%和97.2%,新分离病毒E基因区段与疫苗株存在11处氨基酸位点差异,与2002年在辽宁省分离的乙脑病毒相比,未发现氨基酸位点变异.结论自2002年以来在东港市再次分离到基因1型乙脑病毒,与2002年在辽宁分离的基因1型乙脑病毒相比E基因区段氨基酸未发生变异.基因1型乙脑病毒在辽宁省东港市持续存在.
目的 瞭解2007年在遼寧省分離的乙型腦炎病毒基因型彆及其病毒E基因分子特徵.方法2006年8月在遼寧省東港市採集蚊蟲標本,利用組織培養細胞進行病毒分離,對病毒分離物進行血清學和分子生物學鑒定.結果從採集的30批,共1500隻三帶喙庫蚊標本中分離到2株病毒,命名為LNDG07-02、LNDG07-16,經鑒定均為基因1型乙腦病毒.病毒E基因區段覈苷痠和氨基痠序列與乙腦減毒活疫苗株(SA14-14-2株)的同源性分彆為87.8%～88.0%和97.2%,新分離病毒E基因區段與疫苗株存在11處氨基痠位點差異,與2002年在遼寧省分離的乙腦病毒相比,未髮現氨基痠位點變異.結論自2002年以來在東港市再次分離到基因1型乙腦病毒,與2002年在遼寧分離的基因1型乙腦病毒相比E基因區段氨基痠未髮生變異.基因1型乙腦病毒在遼寧省東港市持續存在.
목적 료해2007년재요녕성분리적을형뇌염병독기인형별급기병독E기인분자특정.방법2006년8월재요녕성동항시채집문충표본,이용조직배양세포진행병독분리,대병독분리물진행혈청학화분자생물학감정.결과종채집적30비,공1500지삼대훼고문표본중분리도2주병독,명명위LNDG07-02、LNDG07-16,경감정균위기인1형을뇌병독.병독E기인구단핵감산화안기산서렬여을뇌감독활역묘주(SA14-14-2주)적동원성분별위87.8%～88.0%화97.2%,신분리병독E기인구단여역묘주존재11처안기산위점차이,여2002년재요녕성분리적을뇌병독상비,미발현안기산위점변이.결론자2002년이래재동항시재차분리도기인1형을뇌병독,여2002년재료녕분리적기인1형을뇌병독상비E기인구단안기산미발생변이.기인1형을뇌병독재요녕성동항시지속존재.
Objective To isolate Japanese Encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene. Methods Collected mosquitoes in Dandong Liaoning province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods. Results Two new JEV strains, LNDG07-02, LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 were 87.8%-88.0% and 97.2% , respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002. Conclusion Genotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning province.
