CAJ | 학술논문

在合适的结晶条件下,从含Cr无氨培养基中生长的固氮菌(Azotobacter vinelandii Lipmann)突变种UW3中纯化出的CrFe蛋白可从溶液中析出深棕色斜四棱柱晶体,晶体最大的两条对角线长度分别可达0.25 mm和0.12 mm.PEG 8000、MgCl2、NaCl、Tris 和Hepes 缓冲液的浓度及结晶方法等对该蛋白的出晶率、晶核数目、晶体大小和质量都有明显影响.CrFe蛋白结晶所需的上述化合物的最适浓度与在Mn中生长的固氮菌突变种UW3的MnFe蛋白和缺失nifZ固氮菌突变种的ΔnifZ MoFe蛋白结晶所需的最适浓度有所不同.结果表明,该蛋白晶体可能为CrFe蛋白的晶体.
재합괄적결정조건하,종함Cr무안배양기중생장적고담균(Azotobacter vinelandii Lipmann)돌변충UW3중순화출적CrFe단백가종용액중석출심종색사사릉주정체,정체최대적량조대각선장도분별가체0.25 mm화0.12 mm.PEG 8000、MgCl2、NaCl、Tris 화Hepes 완충액적농도급결정방법등대해단백적출정솔、정핵수목、정체대소화질량도유명현영향.CrFe단백결정소수적상술화합물적최괄농도여재Mn중생장적고담균돌변충UW3적MnFe단백화결실nifZ고담균돌변충적ΔnifZ MoFe단백결정소수적최괄농도유소불동.결과표명,해단백정체가능위CrFe단백적정체.
Under a suitable condition of crystallization, dark brown rhombohedron crystals (the lengths of the longest two diagonals were 0.25 and 0.12 mm, respectively) could be obtained from nitrogenase CrFe protein purified from a mutant UW3 of Azotobacter vinelandii Lipmann grown in Cr-containing but NH3-free medium. The possibility of crystallization, as well as the number, size and quality of crystals obviously depended on the concentrations of PEG 8000, MgCl2, NaCl, Tris and Hepes buffer, and methods of crystallization. The optimum concentrations of the chemicals for crystallization of CrFe protein were slightly different from those for crystallization of MnFe protein from UW3 grown in Mn and ΔnifZ MoFe protein from a nifZ deleted strain of A. vinelandii. The crystal seemed to be formed from CrFe protein.