CAJ | 학술논문

[目的] 通过实验室细胞染毒模型来揭示无铅汽油的使用能否减低汽车尾气颗粒有机提取物的细胞膜毒性.[方法]在发动机台架试验上,分别使用市售90#含铅及无铅两种汽油来制造尾气.收集尾气的颗粒物及其吸附有机物,并检测尾气中HC与CO浓度.将体外培养中国仓鼠肺细胞(CHL)暴露于尾气的提取物中,通过检测细胞培养液中乳酸脱氢酶(LDH)及细胞内K+及Ca2+浓度来分析细胞内外物质交换的变化,从而说明不同汽油排出尾气颗粒有机提取物的细胞膜毒性.[结果]随着对细胞染毒剂量的增加,培养液中的LDH浓度以及细胞内的Ca2+浓度有增加的趋势,而细胞内K+浓度有降低的趋势,这种变化呈剂量-反应关系.统计学检验表明,无铅汽油尾气颗粒提取物的细胞膜毒性与对照组及其他实验组有显著性的差别.[结论]无铅汽油的使用能减少尾气中CO、HC及颗粒物的排放.与含铅汽油相比较,无铅汽油的颗粒吸附有机物的细胞膜毒性有所降低.
[목적] 통과실험실세포염독모형래게시무연기유적사용능부감저기차미기과립유궤제취물적세포막독성.[방법]재발동궤태가시험상,분별사용시수90#함연급무연량충기유래제조미기.수집미기적과립물급기흡부유궤물,병검측미기중HC여CO농도.장체외배양중국창서폐세포(CHL)폭로우미기적제취물중,통과검측세포배양액중유산탈경매(LDH)급세포내K+급Ca2+농도래분석세포내외물질교환적변화,종이설명불동기유배출미기과립유궤제취물적세포막독성.[결과]수착대세포염독제량적증가,배양액중적LDH농도이급세포내적Ca2+농도유증가적추세,이세포내K+농도유강저적추세,저충변화정제량-반응관계.통계학검험표명,무연기유미기과립제취물적세포막독성여대조조급기타실험조유현저성적차별.[결론]무연기유적사용능감소미기중CO、HC급과립물적배방.여함연기유상비교,무연기유적과립흡부유궤물적세포막독성유소강저.
[Objective] To investigate if use of unleaded gasoline (ULG) has reduced the toxicity of gasoline exhaust particulates (GEPs) to cell membrane in vitro. [Methods] The ULG and leaded gasoline (LG) were used to the Engine Dynamometer during idle and medium-duty operating status,respectively,to produce tail exhausts. The hydrocarbons (HC) and carbon monoxide (CO) of the exhausts were detected. The GEPs were collected to extract absorbed organic matter. The in vitro cultured Chinese hamster lung (CHL) cells were treated with different extracts. The quantity of cytosolic lactate dehydrogenase (LDH) released from the cells into the media,and the intracellular concentrations of K+ and Ca2+ were detected to assess the degree of toxic damage to the cell membrane. [Results] Both LDH content in the media and intracellular Ca2+ concentration increased,while intracellular K+ content decreased accordingly. These changes showed a dose-response relationship,suggesting that GEPs damaged cell membrane,resulting in an outflow of intracellular LDH and K+ from the cells,and an inflow of extracellular Ca2+ into the cells. Statistically significant differences in cell toxicity was found between the cells treated with ULG GEPs extract and others. [Conclusion] ULG has reduced the emission of hydrocarbons (HC) and carbon monoxide (CO). The GEPs from the ULG present less cell membrane toxicity than those from the LG.