CAJ | 학술논문

目的是以碳酸酐酶II(CA II)为靶点筛选其抑制剂,以期寻找抗骨质疏松活性样品.实验是在96孔酶标板上对来源于178个科、608个属、1020种动植物2919个提取物或分离部位样品在CA-II模型上进行了批量筛选.结果表明在10μg/ml浓度下发现了来源于40个科、61个属、72个种的100个样品有活性,其中5个样品的IC50小于2.50μg/ml,22个样品的IC50在2.51～5.00μg/ml范围,73个样品的IC50在5.01～10.00μg/ml范围.通过以上工作我们认为以碳酸酐酶II为分子靶点的体外筛选方法稳定、方便、快速、微量、有效,特别适用于天然产物的抗骨质疏松活性筛选.
목적시이탄산항매II(CA II)위파점사선기억제제,이기심조항골질소송활성양품.실험시재96공매표판상대래원우178개과、608개속、1020충동식물2919개제취물혹분리부위양품재CA-II모형상진행료비량사선.결과표명재10μg/ml농도하발현료래원우40개과、61개속、72개충적100개양품유활성,기중5개양품적IC50소우2.50μg/ml,22개양품적IC50재2.51～5.00μg/ml범위,73개양품적IC50재5.01～10.00μg/ml범위.통과이상공작아문인위이탄산항매II위분자파점적체외사선방법은정、방편、쾌속、미량、유효,특별괄용우천연산물적항골질소송활성사선.
To discover inhibitors of carbonic anhydrase II (CA II) with antiosteoporotic activity a batch of 2919 extract or fraction samples prepared from plants and animals belonging to 178 families,608 genera and 1020 species were tested on carbonic anhydrase II bioassay (CA-II) in 96-well microtiterplates.Finally 100 samples, which belong to 40 families, 61 genera and 72 species, showed inhibitory activity at 10 μg/ml, in which 5 samples showed IC50 at =<2.50 μg/ml,22 samples showed IC50 at 2.51-5.00 μg/ml and 73 samples showed IC50 at 5.01-10.00 μg/ml.It is indicated that this in-vitro bioassay is convenient,stable,rapid,sensitive and effective in searching for antiosteoporotic activity samples from natural sources.