背景:天麻素广泛应用于眩晕、头痛及高血压等的辅助治疗,其作为干预物质用于高脂肥胖大鼠的治疗有待评估.目的:构建不同浓度天麻素对高脂饮食性肥胖大鼠模型,观察其对体质量与血清代谢物水平的影响,分析其可能的作用机制.设计:随机对照动物实验.单位:兰州大学基础医学院生理与心理研究所.材料:实验于2007-06/2007-08在兰州大学基础医学院生理与心理研究所和甘肃省新药临床前研究重点实验室完成.选用44只生后1周雄性健康SD大鼠,体质量(99.57±2.13)g,由中科院上海斯莱克实验动物有限公司提供.实验过程中对动物的处置符合动物伦理学标准.基础饲料由苏州双狮实验动物饲料科技有限公司生产,高脂饲料由本实验室自行配制.每100克含基础饲料57.5 g、蛋黄粉11.79g、猪油10g、猪胆盐0.2g、酪蛋白7g、奶粉13g、食盐0.085g、酵母粉0.425g;其中含脂肪22.07g、蛋白质23.7g、碳水化合物39g、热量472.16千卡.纯度为98%的天麻素购自陕西旭煌植物科技开发公司.丙二醛和总抗氧化能力试剂盒均为南京建成生物工程研究所生产.方法:构建天麻素干预高脂饮食性肥胖大鼠模型:①采用基础饲料适应喂养大鼠1周,随机数字表法将大鼠分为正常对照组(n=10)、高脂组(n=10)、高脂+天麻素低、中、高剂量组(每剂量组各8只).②正常对照组喂基础饲料,其它各组均喂高脂饲料.正常对照组和高脂组每天灌胃0.9%生理盐水0.3 mL,高脂+天麻素低、中、高组灌胃同等容量浓度分别为15,30,60mg/(kg·d)的天麻素溶液.③各组大鼠自由进食和饮水,共8周.主要观察指标:①每周末检测各组大鼠体质量.②每日上午定时记录大鼠摄入热量.③高脂喂养结束后,于大鼠股动脉取血测血糖值,同时检测血清丙二醛、总抗氧化能力、胰岛素、游离脂肪酸,血脂、谷丙转氨酶及谷草转氨酶水平,并计算胰岛素敏感指数和抵抗指数.结果:大鼠44只均进入结果分析.①体质量:高脂喂养4～8周高脂组大鼠体质量明显高于正常对照组,差异有显著性意义(P＜0.001).天麻素各剂量组体质量均低于高脂组,差异有显著性意义(P＜0.05～0.01),高脂+天麻素低、中、高剂量组组间比较,差异无显著性意义(P＞0.05),提示天麻素抑制大鼠高脂饮食肥胖无剂量相关性.②摄入热量:高脂组大鼠高于正常对照组,差异有显著性意义(P＜0.01).高脂饮食4周后天麻素各剂量组均低于高脂组,差异有显著性意义(P＜0.05～0.01).③血清丙二醛、总抗氧化能力、谷丙转氨酶、谷草转氨酶水平:与正常对照组相比,高脂组的总抗氧化能力下降,丙二醛水平上升,谷丙转氨酶水平增加,差异均有显著性意义(P＜0.01).与高脂组相比,天麻素低剂量组的总抗氧化能力明显上升,丙二醛水平下降,差异均有显著性意义(P＜0.01).④胰岛素及血糖水平:与正常对照组比较,高脂组大鼠血糖浓度升高,胰岛素敏感指数下降,抵抗指数上升,差异有显著性意义(P＜0.05～0.01).与高脂组比较,天麻素低剂量组血糖浓度降低,胰岛素敏感指数升高,胰岛素抵抗指数降低,差异均有显著性意义(P＜0.05～0.01).⑤游离脂肪酸及血脂水平:与正常对照组比较,高脂组大鼠的游离脂肪酸、低密度脂蛋白胆固醇升高,高密度脂蛋白胆固醇降低,差异有显著性(P＜0.05～0.01).结论:天麻素可抑制高脂饮食性肥胖大鼠体质量增加,作用机理可能与其调节葡萄糖和游离脂肪酸代谢、改善胰岛素抵抗和提高抗氧化能力有关.
揹景:天痳素廣汎應用于眩暈、頭痛及高血壓等的輔助治療,其作為榦預物質用于高脂肥胖大鼠的治療有待評估.目的:構建不同濃度天痳素對高脂飲食性肥胖大鼠模型,觀察其對體質量與血清代謝物水平的影響,分析其可能的作用機製.設計:隨機對照動物實驗.單位:蘭州大學基礎醫學院生理與心理研究所.材料:實驗于2007-06/2007-08在蘭州大學基礎醫學院生理與心理研究所和甘肅省新藥臨床前研究重點實驗室完成.選用44隻生後1週雄性健康SD大鼠,體質量(99.57±2.13)g,由中科院上海斯萊剋實驗動物有限公司提供.實驗過程中對動物的處置符閤動物倫理學標準.基礎飼料由囌州雙獅實驗動物飼料科技有限公司生產,高脂飼料由本實驗室自行配製.每100剋含基礎飼料57.5 g、蛋黃粉11.79g、豬油10g、豬膽鹽0.2g、酪蛋白7g、奶粉13g、食鹽0.085g、酵母粉0.425g;其中含脂肪22.07g、蛋白質23.7g、碳水化閤物39g、熱量472.16韆卡.純度為98%的天痳素購自陝西旭煌植物科技開髮公司.丙二醛和總抗氧化能力試劑盒均為南京建成生物工程研究所生產.方法:構建天痳素榦預高脂飲食性肥胖大鼠模型:①採用基礎飼料適應餵養大鼠1週,隨機數字錶法將大鼠分為正常對照組(n=10)、高脂組(n=10)、高脂+天痳素低、中、高劑量組(每劑量組各8隻).②正常對照組餵基礎飼料,其它各組均餵高脂飼料.正常對照組和高脂組每天灌胃0.9%生理鹽水0.3 mL,高脂+天痳素低、中、高組灌胃同等容量濃度分彆為15,30,60mg/(kg·d)的天痳素溶液.③各組大鼠自由進食和飲水,共8週.主要觀察指標:①每週末檢測各組大鼠體質量.②每日上午定時記錄大鼠攝入熱量.③高脂餵養結束後,于大鼠股動脈取血測血糖值,同時檢測血清丙二醛、總抗氧化能力、胰島素、遊離脂肪痠,血脂、穀丙轉氨酶及穀草轉氨酶水平,併計算胰島素敏感指數和牴抗指數.結果:大鼠44隻均進入結果分析.①體質量:高脂餵養4～8週高脂組大鼠體質量明顯高于正常對照組,差異有顯著性意義(P＜0.001).天痳素各劑量組體質量均低于高脂組,差異有顯著性意義(P＜0.05～0.01),高脂+天痳素低、中、高劑量組組間比較,差異無顯著性意義(P＞0.05),提示天痳素抑製大鼠高脂飲食肥胖無劑量相關性.②攝入熱量:高脂組大鼠高于正常對照組,差異有顯著性意義(P＜0.01).高脂飲食4週後天痳素各劑量組均低于高脂組,差異有顯著性意義(P＜0.05～0.01).③血清丙二醛、總抗氧化能力、穀丙轉氨酶、穀草轉氨酶水平:與正常對照組相比,高脂組的總抗氧化能力下降,丙二醛水平上升,穀丙轉氨酶水平增加,差異均有顯著性意義(P＜0.01).與高脂組相比,天痳素低劑量組的總抗氧化能力明顯上升,丙二醛水平下降,差異均有顯著性意義(P＜0.01).④胰島素及血糖水平:與正常對照組比較,高脂組大鼠血糖濃度升高,胰島素敏感指數下降,牴抗指數上升,差異有顯著性意義(P＜0.05～0.01).與高脂組比較,天痳素低劑量組血糖濃度降低,胰島素敏感指數升高,胰島素牴抗指數降低,差異均有顯著性意義(P＜0.05～0.01).⑤遊離脂肪痠及血脂水平:與正常對照組比較,高脂組大鼠的遊離脂肪痠、低密度脂蛋白膽固醇升高,高密度脂蛋白膽固醇降低,差異有顯著性(P＜0.05～0.01).結論:天痳素可抑製高脂飲食性肥胖大鼠體質量增加,作用機理可能與其調節葡萄糖和遊離脂肪痠代謝、改善胰島素牴抗和提高抗氧化能力有關.
배경:천마소엄범응용우현훈、두통급고혈압등적보조치료,기작위간예물질용우고지비반대서적치료유대평고.목적:구건불동농도천마소대고지음식성비반대서모형,관찰기대체질량여혈청대사물수평적영향,분석기가능적작용궤제.설계:수궤대조동물실험.단위:란주대학기출의학원생리여심리연구소.재료:실험우2007-06/2007-08재란주대학기출의학원생리여심리연구소화감숙성신약림상전연구중점실험실완성.선용44지생후1주웅성건강SD대서,체질량(99.57±2.13)g,유중과원상해사래극실험동물유한공사제공.실험과정중대동물적처치부합동물윤리학표준.기출사료유소주쌍사실험동물사료과기유한공사생산,고지사료유본실험실자행배제.매100극함기출사료57.5 g、단황분11.79g、저유10g、저담염0.2g、락단백7g、내분13g、식염0.085g、효모분0.425g;기중함지방22.07g、단백질23.7g、탄수화합물39g、열량472.16천잡.순도위98%적천마소구자협서욱황식물과기개발공사.병이철화총항양화능력시제합균위남경건성생물공정연구소생산.방법:구건천마소간예고지음식성비반대서모형:①채용기출사료괄응위양대서1주,수궤수자표법장대서분위정상대조조(n=10)、고지조(n=10)、고지+천마소저、중、고제량조(매제량조각8지).②정상대조조위기출사료,기타각조균위고지사료.정상대조조화고지조매천관위0.9%생리염수0.3 mL,고지+천마소저、중、고조관위동등용량농도분별위15,30,60mg/(kg·d)적천마소용액.③각조대서자유진식화음수,공8주.주요관찰지표:①매주말검측각조대서체질량.②매일상오정시기록대서섭입열량.③고지위양결속후,우대서고동맥취혈측혈당치,동시검측혈청병이철、총항양화능력、이도소、유리지방산,혈지、곡병전안매급곡초전안매수평,병계산이도소민감지수화저항지수.결과:대서44지균진입결과분석.①체질량:고지위양4～8주고지조대서체질량명현고우정상대조조,차이유현저성의의(P＜0.001).천마소각제량조체질량균저우고지조,차이유현저성의의(P＜0.05～0.01),고지+천마소저、중、고제량조조간비교,차이무현저성의의(P＞0.05),제시천마소억제대서고지음식비반무제량상관성.②섭입열량:고지조대서고우정상대조조,차이유현저성의의(P＜0.01).고지음식4주후천마소각제량조균저우고지조,차이유현저성의의(P＜0.05～0.01).③혈청병이철、총항양화능력、곡병전안매、곡초전안매수평:여정상대조조상비,고지조적총항양화능력하강,병이철수평상승,곡병전안매수평증가,차이균유현저성의의(P＜0.01).여고지조상비,천마소저제량조적총항양화능력명현상승,병이철수평하강,차이균유현저성의의(P＜0.01).④이도소급혈당수평:여정상대조조비교,고지조대서혈당농도승고,이도소민감지수하강,저항지수상승,차이유현저성의의(P＜0.05～0.01).여고지조비교,천마소저제량조혈당농도강저,이도소민감지수승고,이도소저항지수강저,차이균유현저성의의(P＜0.05～0.01).⑤유리지방산급혈지수평:여정상대조조비교,고지조대서적유리지방산、저밀도지단백담고순승고,고밀도지단백담고순강저,차이유현저성(P＜0.05～0.01).결론:천마소가억제고지음식성비반대서체질량증가,작용궤리가능여기조절포도당화유리지방산대사、개선이도소저항화제고항양화능력유관.
BACKGROUND: Gastrodin (GAS) is widely used as adjuvant therapy for vertigo, headache and hypertension. However, it is recently noticed that GAS might be used as an agent for treating obesity.OBJECTIVE: To set up obese rats of high-fat diet to observe the effects of different concentrations of GAS on body mass and serum metabolite levels and to analyze its possible mechanism.DESIGN: A randomized and controlled animal experiment.SETTING: Institute of Physiology and Psychology, School of Basic Medical Science, Lanzhou University.MATERIALS: This study was performed at the Institute of Physiology and Psychology, School of Basic Medical Science, Lanzhou University and Gansu Provincial Key Laboratory of Pre-clinical Study for New Drugs from June to August in 2007. Forty-four healthy one-week-old male SD rats, weighing (99.57±2.13)g, were purchased from Shanghai SILAIKE Laboratory Animal Co., Ltd. Disposal of animals was in accordance with the animal ethics standards. Basic animal feed was provided by Suzhou Shuangshi Laboratory Animal Feed Science and Technology Co., Ltd. High-fat forage were self-made in the authors' laboratory. Each 100 gram of high-fat forage consisted of basic feed (57.5g), egg yolk powder (11.79g), lard (10g), pig bile salt (0.2g), casein (7g), milk power (13g), salt(0.085g), and yeast powder (0.425g), and the 100 gram of high-fat forage contained of fat (22.07g), protein (23.7g), carbohydrate (39g), and quantity of heat (472.16 calorie). GAS (98% in purity) was purchased from Shaanxi Xuhuang Botanical Science & Technology Development Co., Ltd. Malondialdehyde (MDA) and total antioxidative capability (T-AOC) kits were purchased from Jiancheng Bioengineering Institute, Nanjng, Jiangsu Province.MAIN OUTCOME MEASURES: The body mass was measured every seven days. The food intake in each group was monitored in every morning. At the end of the experiment, femoral artery blood samples were collected to determine the blood glucose, the serum levels of MDA, T-AOC, Insulin, free fatty acid (FFA), glutamate-pyruvate transaminase (GPT), glutamic oxalacetic transaminase (GOT) and blood lipid profile. Insulin resistance index (IRI) and insulin sensitivity index (ISI) were calculated as IRI=(FBG×FINS)/22.5 and ISI=1/(FINS×FBG).RESULTS: All 44 rats were included in the final analysis. Body mass: The body mass in the HFFC group was significantly higher than in the NC group from 4th-8th weeks (P＜0.01), while the body mass in GAS groups was lower compared to HFFC group (P＜0.05-0.01). There were no significant differences among the GAS-H, GAS-M, and GAS-L groups (P＞0.05). Therefore, GAS had no dose-dependent relationship in inhibiting the body mass of obese rats of high-fat diet. Caloric intake: The caloric intake was significantly higher in the HFFC group than in the NC group (P＜0.01), and was significantly decreased in GAS group compared to NFFC group from the 4th week (P＜0.05-0.01). Serum levels of MDA, T-AOC, GPT and GOT: The serum level of T-AOC was decreased and that of MDA, GPT were increased significantly in the HFFC group compared with NC group (P＜0.01, P＜0.05). In the GAS-L group, T-AOC, level was significantly increased and MDA level was significantly decreased compared to HFFC group (both P＜0.01). Levels of blood glucose and insulin: In the HFFC group, blood glucose level and IRI were significantly increased, and ISI was obviously decreased compared to NC group (P＜0.05-0.01). In the GAS-L group, blood glucose level and IRI were significantly decreased, and ISI was significantly increased compared to HFFC group (P＜0.05-0.01). FFA and lipoprotein cholesterol levels: In the HFFC group, FFA and low-density lipoprotein cholesterol levels were increased and high-density lipoprotein cholesterol level was decreased compared to NC group (P＜0.05-0.01).CONCLUSION: GAS may play an important role in inhibiting rats' body mass of high-fat diet. The mechanism of action may be related to GAS regulating the metabolism of blood glucose and FFA, improving IRI and elevating T-AOC.
