中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2009年
10期
892-895
,共4页
马荣%谢学军%万李%马殿伟
馬榮%謝學軍%萬李%馬殿偉
마영%사학군%만리%마전위
高糖%糖波动%视网膜神经节细胞%乳酸脱氢酶%补肾活血
高糖%糖波動%視網膜神經節細胞%乳痠脫氫酶%補腎活血
고당%당파동%시망막신경절세포%유산탈경매%보신활혈
high glucose%glucose fluctuation%retinal ganglion cell%lactate dehydrogenase%nourishing Shen and activating blood
目的 探讨补肾活血中药血清对体外高糖状态下纯化培养的视网膜神经节细胞活力的影响.方法 体外纯化SD大鼠视网膜神经节细胞(retinal ganglion cells,RGCs),模拟稳定高糖(50 mmol/L)及糖波动环境进行培养,以补肾活血中药血清进行干预,检测RGCs乳酸脱氢酶(lactate dehydrogenase,LDH)漏出量,以推测RGCs活力.结果 RGCs的LDH漏出量(U/L)稳定高糖组24 h(1 349.17±215.50)、48 h(1 220.24±124.53)及72 h(1 982.14±219.03)均较正常对照组(1 628.10±122.10、1 484.13±127.55及2 155.75±140.44)降低(P<0.05);而糖波动组的LDH漏出量在72 h(2 299.60±88.35)较正常对照组增加(P<0.05),且糖波动组各时段的LDH漏出量均较稳定高糖组增加(P<0.05);稳定高糖中药干预组的LDH漏出量在72 h(1 797.62±146.40)时较稳定高糖组减少(P<0.05);糖波动中药干预组的LDH漏出量在48 h(1 259.92±87.74)和72 h(1 940.40±155.47)时均较糖波动组减少(P<0.05).结论 糖波动能明显降低RGCs细胞膜的稳定性,增加细胞膜通透性,降低细胞活力;补肾活血中药血清能降低本实验中稳定高糖及糖波动条件下RGCs的细胞膜通透性,提高细胞膜稳定性,增强其细胞活力,这可能是补肾活血中药复方防治糖尿病视网膜病变(diabetic retinopathy,DRP)的药物干预途径之一.
目的 探討補腎活血中藥血清對體外高糖狀態下純化培養的視網膜神經節細胞活力的影響.方法 體外純化SD大鼠視網膜神經節細胞(retinal ganglion cells,RGCs),模擬穩定高糖(50 mmol/L)及糖波動環境進行培養,以補腎活血中藥血清進行榦預,檢測RGCs乳痠脫氫酶(lactate dehydrogenase,LDH)漏齣量,以推測RGCs活力.結果 RGCs的LDH漏齣量(U/L)穩定高糖組24 h(1 349.17±215.50)、48 h(1 220.24±124.53)及72 h(1 982.14±219.03)均較正常對照組(1 628.10±122.10、1 484.13±127.55及2 155.75±140.44)降低(P<0.05);而糖波動組的LDH漏齣量在72 h(2 299.60±88.35)較正常對照組增加(P<0.05),且糖波動組各時段的LDH漏齣量均較穩定高糖組增加(P<0.05);穩定高糖中藥榦預組的LDH漏齣量在72 h(1 797.62±146.40)時較穩定高糖組減少(P<0.05);糖波動中藥榦預組的LDH漏齣量在48 h(1 259.92±87.74)和72 h(1 940.40±155.47)時均較糖波動組減少(P<0.05).結論 糖波動能明顯降低RGCs細胞膜的穩定性,增加細胞膜通透性,降低細胞活力;補腎活血中藥血清能降低本實驗中穩定高糖及糖波動條件下RGCs的細胞膜通透性,提高細胞膜穩定性,增彊其細胞活力,這可能是補腎活血中藥複方防治糖尿病視網膜病變(diabetic retinopathy,DRP)的藥物榦預途徑之一.
목적 탐토보신활혈중약혈청대체외고당상태하순화배양적시망막신경절세포활력적영향.방법 체외순화SD대서시망막신경절세포(retinal ganglion cells,RGCs),모의은정고당(50 mmol/L)급당파동배경진행배양,이보신활혈중약혈청진행간예,검측RGCs유산탈경매(lactate dehydrogenase,LDH)루출량,이추측RGCs활력.결과 RGCs적LDH루출량(U/L)은정고당조24 h(1 349.17±215.50)、48 h(1 220.24±124.53)급72 h(1 982.14±219.03)균교정상대조조(1 628.10±122.10、1 484.13±127.55급2 155.75±140.44)강저(P<0.05);이당파동조적LDH루출량재72 h(2 299.60±88.35)교정상대조조증가(P<0.05),차당파동조각시단적LDH루출량균교은정고당조증가(P<0.05);은정고당중약간예조적LDH루출량재72 h(1 797.62±146.40)시교은정고당조감소(P<0.05);당파동중약간예조적LDH루출량재48 h(1 259.92±87.74)화72 h(1 940.40±155.47)시균교당파동조감소(P<0.05).결론 당파동능명현강저RGCs세포막적은정성,증가세포막통투성,강저세포활력;보신활혈중약혈청능강저본실험중은정고당급당파동조건하RGCs적세포막통투성,제고세포막은정성,증강기세포활력,저가능시보신활혈중약복방방치당뇨병시망막병변(diabetic retinopathy,DRP)적약물간예도경지일.
Objective To investigate the effect of serum contained Chinese drugs for nourishing Shen and activating blood(S-NSAB)on activity of purified retinal ganglion cells(RGCs)cultured in high glucose medium.Methods Purified RGCs of SD rats were cultured in simulative stable high glucose(50 mmol/L)condition(SHG)and fluctuated glucose condition(FGC)separately,they were intervened with S-NSAB,and the lactate dehydrogenase(LDH)leakage was detected by spectrophotometer for estimating the activity of RGCs.Results LDH leakage(U/L)in SHG culture was 1 349.17±215.50 at 24 h,1 220.24±124.53 at 48 h and 1 982.14±219.03 at 72 h,all significantly lower than that in normal control at the corresponding time points(1 628.10±122.10,1 484.13±127.55 and 2 155.75±140.44,respectively,P<0.05),whereas it was obviously higher in FGC culture at 72 h(2 299.60±88.35),showing that LDH leakage in FGC was significantly higher than that in SHG at the corresponding time points(P<0.05).The LDH leakage was obviously decreased by Chinese medicine intervention with S-NSAB both in SHG at 72 h(1 797.62±146.40)and in FGC at 48 h(1 259.92±87.74)and 72 h(1 940.40±155.47),the difference between pre-and post-intervention was significant(P<0.05).Conclusion Fluctuated glucose conditions of culture medium could obviously damage the membranous stability of RGCs to enhance their permeability and lower the activity of cells;S-NSAB could improve these abnormalities in either SHG or FGC condition,which may be one of the important mechanisms of Chinese formula for nourishing Shen and activating blood in preventing and treating diabetic retinopathy.
