CAJ | 학술논문

利用聚丙烯酰胺垂直板电泳技术对3个睡莲Nymphaea品种'彼得''Peter Slocum','玛珊姑娘''Masaniello'和'得克萨斯''Texas Dawn'不同器官(未展开的幼叶、展开的幼叶、老叶、花辩、花蕾、叶柄)和未展开的幼叶在营养生长阶段和生殖生长阶段的过氧化物酶(POD)争酯酶(EST)同工酶进行了研究.结果表明:①3个品种共有12种过氧化物酶酶带,6种酯酶酶带.②睡莲的过氧化物酶和酯酶具有组织特异性,其中未展开嫩叶和展开嫩叶的酶带最多,叶柄和花蕾次之,花瓣和老叶最少.未展开幼叶为较适合用于睡莲同工酶分析.③与生殖生长阶段相比,3个睡莲品种未展开幼叶在营养生长阶段的酯酶表达量更大,条带更多;而过氧化物酶同工酶表达量则是生殖生长阶段的表达量大,但3个品种的酶条带变化不一致.其中'彼得'和'玛珊姑娘'在生殖生长阶段的过氧化物酶同工酶阶段条带多'得克萨斯'则相反.
이용취병희선알수직판전영기술대3개수련Nymphaea품충'피득''Peter Slocum','마산고낭''Masaniello'화'득극살사''Texas Dawn'불동기관(미전개적유협、전개적유협、로협、화변、화뢰、협병)화미전개적유협재영양생장계단화생식생장계단적과양화물매(POD)쟁지매(EST)동공매진행료연구.결과표명:①3개품충공유12충과양화물매매대,6충지매매대.②수련적과양화물매화지매구유조직특이성,기중미전개눈협화전개눈협적매대최다,협병화화뢰차지,화판화로협최소.미전개유협위교괄합용우수련동공매분석.③여생식생장계단상비,3개수련품충미전개유협재영양생장계단적지매표체량경대,조대경다;이과양화물매동공매표체량칙시생식생장계단적표체량대,단3개품충적매조대변화불일치.기중'피득'화'마산고낭'재생식생장계단적과양화물매동공매계단조대다'득극살사'칙상반.
Peroxidase isozyme(POD)and esterase isozyme(EST)of three water lily cultivars,Nymphaea'Peter Slocum',N.'Masaniello',and N.'Texas Dawn',were studied using polyacrylamide gel electrophoresis(PAGE)in different organs(closed young leaf,open young leaf,old leaf,petal,flower bud and petiole)and growth stages of a closed young leaf.Results showed(1)12 POD bands and 6 EST bands.Also,(2)POD and EST were tissue specific for the closed young leaf,open young leaf,old leaf,petal,bud,and petiole.The most isozyme bands were observed in the closed young leaf and the open young leaf with the least in the old leaf and petal.(3)In addition,compared to the reproductive growth stage,the closed leaf in the vegetative growth stage had more EST and EST bands.Meanwhile,there was more POD during the reproductive stage.However,during the reproductive growth stage more POD bands of N.'Peter Slocum'and N.'Masaniello'were found with more POD bands of N.'Texas Dawn'noted during the vegetative growth stage.