中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2001年
2期
105-108
,共4页
哇巴因%地高辛%心肌%基因
哇巴因%地高辛%心肌%基因
왜파인%지고신%심기%기인
目的 对比研究哇巴因与地高辛对大鼠心肌钠泵(Na+-K+-ATP酶)α亚单位基因表达的影响。方法 长期给予大鼠注射小剂量哇巴因(20 μg*kg-1*d-1)与地高辛(32 μg*kg-1*d-1)，分别应用分子生物学RT-PCR及免疫组织化学技术分析大鼠心肌钠泵α1、α2及α3亚单位mRNA及蛋白水平基因表达的改变。结果 长期给予大鼠注射小剂量哇巴因可使大鼠血压升高，而地高辛对大鼠血压无影响。无论是在mRNA水平还是在蛋白水平，哇巴因组大鼠心肌钠泵α1亚单位表达减弱，α3亚单位表达增强，而α2亚单位表达无改变；地高辛组大鼠心肌钠泵α3亚单位表达增强，而α1与α2亚单位表达无改变。结论 哇巴因在高血压发病中可能起着重要作用；哇巴因与地高辛可导致不同的钠泵基因表达改变。
目的 對比研究哇巴因與地高辛對大鼠心肌鈉泵(Na+-K+-ATP酶)α亞單位基因錶達的影響。方法 長期給予大鼠註射小劑量哇巴因(20 μg*kg-1*d-1)與地高辛(32 μg*kg-1*d-1)，分彆應用分子生物學RT-PCR及免疫組織化學技術分析大鼠心肌鈉泵α1、α2及α3亞單位mRNA及蛋白水平基因錶達的改變。結果 長期給予大鼠註射小劑量哇巴因可使大鼠血壓升高，而地高辛對大鼠血壓無影響。無論是在mRNA水平還是在蛋白水平，哇巴因組大鼠心肌鈉泵α1亞單位錶達減弱，α3亞單位錶達增彊，而α2亞單位錶達無改變；地高辛組大鼠心肌鈉泵α3亞單位錶達增彊，而α1與α2亞單位錶達無改變。結論 哇巴因在高血壓髮病中可能起著重要作用；哇巴因與地高辛可導緻不同的鈉泵基因錶達改變。
목적 대비연구왜파인여지고신대대서심기납빙(Na+-K+-ATP매)α아단위기인표체적영향。방법 장기급여대서주사소제량왜파인(20 μg*kg-1*d-1)여지고신(32 μg*kg-1*d-1)，분별응용분자생물학RT-PCR급면역조직화학기술분석대서심기납빙α1、α2급α3아단위mRNA급단백수평기인표체적개변。결과 장기급여대서주사소제량왜파인가사대서혈압승고，이지고신대대서혈압무영향。무론시재mRNA수평환시재단백수평，왜파인조대서심기납빙α1아단위표체감약，α3아단위표체증강，이α2아단위표체무개변；지고신조대서심기납빙α3아단위표체증강，이α1여α2아단위표체무개변。결론 왜파인재고혈압발병중가능기착중요작용；왜파인여지고신가도치불동적납빙기인표체개변。
Objective To compare the effects of ouabain and digoxin on both the systolic blood pressure (SBP) and sodium pump α-subunit expression in myocardium of rats. Methods Normal Sprague-Dawley (SD) rats were injected with ouabain, digoxin or normal saline (NS) everyday and indirect SBP was recorded once a week. Six weeks later, all the rats were killed and sodium pump α1-, α2-, and α3-subunit were detected in the left ventricular myocardium with RT-PCR method and immunohistochemical assay at mRNA and protein levels. Results At the end of six weeks SBP of rats infused with ouabain increased significantly (132.6±9.0 vs 115.7±8.2 mm Hg， P＜0.01), while no difference of SBP was found between digoxin group and NS group. The effects of ouabain and digoxin on sodium pump α-subunit isoform expression in myocardium were also different: both ouabain and digoxin stimulated expression of α3-isoform whereas α2-isoform unchanged at both mRNA and protein levels. α1-isoform was decreased in ouabain group and α1-isoform unchanged in digoxin group at both mRNA and protein levels. Conclusion It is suggested that both ouabain and digoxin could regulate sodium pump α-subunit isoform expression, which might be related to the physiological roles of endogenous ouabain and might be responsible for the difference between the pharmacological and toxicological effects of ouabain and digoxin, including their effects on blood pressure.
