中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2008年
6期
390-394
,共5页
万川%徐晨%王琳%李甘地%江炜%张文燕%唐源%阚蓓%刘卫平
萬川%徐晨%王琳%李甘地%江煒%張文燕%唐源%闞蓓%劉衛平
만천%서신%왕림%리감지%강위%장문연%당원%감배%류위평
淋巴瘤,T细胞%皮肤肿瘤%脂膜炎%免疫表型%基因重排
淋巴瘤,T細胞%皮膚腫瘤%脂膜炎%免疫錶型%基因重排
림파류,T세포%피부종류%지막염%면역표형%기인중배
Lymphoma,T-cell%Skin neoplasm%Pannieulitis%Inununophenotyping%Gene rearrangement
目的 探讨免疫表型和基因重排检测在皮下脂膜炎样T细胞淋巴瘤(SPTL)的诊断和鉴别诊断中的意义.方法 参照2005年世界卫生组织-欧洲癌症研究与治疗组织(WHO-EORTC)皮肤淋巴瘤分类标准收集20例SPTL.采用10种抗原标记进行免疫表型检测,运用PCR技术检测TCRγ、IgH基因重排,并用EB病毒编码的小RNA1/2(EBER1/2)原位杂交检测EB病毒感染.结果 (1)本组病例中男9例、女11例,平均年龄29.5岁;(2)所有病例的瘤细胞均表达1个或多个T细胞分化抗原(CD2、CD3或CD45RO),18/19病例表达BF1,18/20病例表达CD8,20/20、16/20病例分别表达细胞毒颗粒相关蛋白TIA-1、颗粒酶B,瘤细胞不表达CD4、CD20和CD56;(3)16/20病例检出TcRγ基因重排,未检出IgH基因重排;(4)5/20病例EBERl/2原位杂交阳性.结论 SPTL的瘤细胞具有克隆性TCR基因重排,综合临床病理、免疫表型及基因重排检测有助于本病确诊.
目的 探討免疫錶型和基因重排檢測在皮下脂膜炎樣T細胞淋巴瘤(SPTL)的診斷和鑒彆診斷中的意義.方法 參照2005年世界衛生組織-歐洲癌癥研究與治療組織(WHO-EORTC)皮膚淋巴瘤分類標準收集20例SPTL.採用10種抗原標記進行免疫錶型檢測,運用PCR技術檢測TCRγ、IgH基因重排,併用EB病毒編碼的小RNA1/2(EBER1/2)原位雜交檢測EB病毒感染.結果 (1)本組病例中男9例、女11例,平均年齡29.5歲;(2)所有病例的瘤細胞均錶達1箇或多箇T細胞分化抗原(CD2、CD3或CD45RO),18/19病例錶達BF1,18/20病例錶達CD8,20/20、16/20病例分彆錶達細胞毒顆粒相關蛋白TIA-1、顆粒酶B,瘤細胞不錶達CD4、CD20和CD56;(3)16/20病例檢齣TcRγ基因重排,未檢齣IgH基因重排;(4)5/20病例EBERl/2原位雜交暘性.結論 SPTL的瘤細胞具有剋隆性TCR基因重排,綜閤臨床病理、免疫錶型及基因重排檢測有助于本病確診.
목적 탐토면역표형화기인중배검측재피하지막염양T세포림파류(SPTL)적진단화감별진단중적의의.방법 삼조2005년세계위생조직-구주암증연구여치료조직(WHO-EORTC)피부림파류분류표준수집20례SPTL.채용10충항원표기진행면역표형검측,운용PCR기술검측TCRγ、IgH기인중배,병용EB병독편마적소RNA1/2(EBER1/2)원위잡교검측EB병독감염.결과 (1)본조병례중남9례、녀11례,평균년령29.5세;(2)소유병례적류세포균표체1개혹다개T세포분화항원(CD2、CD3혹CD45RO),18/19병례표체BF1,18/20병례표체CD8,20/20、16/20병례분별표체세포독과립상관단백TIA-1、과립매B,류세포불표체CD4、CD20화CD56;(3)16/20병례검출TcRγ기인중배,미검출IgH기인중배;(4)5/20병례EBERl/2원위잡교양성.결론 SPTL적류세포구유극륭성TCR기인중배,종합림상병리、면역표형급기인중배검측유조우본병학진.
Objective To explore the diagnostic implication of immunophenotyping and gene rearrangement in subcutaneous panniculitis-like T-cell lymphoma(SPTL).Methods According to the selection criteria of 2005 WHO-EORTC classification for cutaneous lymphomas,20 SPIL patients were enrolled in this study.A 10-antibody panel was used for immunophenotyping and in addition,polymerase chain reaction for TCRγ and IgH gene rearrangement and in situ hybridization for EBER1/2 were also employed. Results There were 9 males and 11 female with a mean age of 29.5 years.Immunophenotypic study showed that all the patients expressed one to three T-cell associated antigens(CD2,CD3 or CD45RO),18 patients were positive for BF1,18 for CD8,20 for TIA-1 and 16 for granzyme B.None of the patients expressed CD4,CD20 and CD56.TCRγ gene rearrangement was found in 16 of 20 cases(80.0%)and none for IgH gene rearrangement.The positive rate of EBER1/2 Was 25.0%(5/20).Conclusions Since the majority of SPTL patients show clonal TCR gene rearrangements,correlations among clinical presentation,histological features,immunophenotype and gene rearrangement data are considered important in confirming a diagnosis of SPTL.