CAJ | 학술논문

目的探讨不同吸入麻醉药对人精子运动功能和体外获能的影响.方法成年男性精液经Percoll梯度离心法处理后,沉淀精子置于人精子体外获能培养基,调节精子密度(30～50)×106/ml,随机分为5组(n=7):对照组(C组)正常培养,2%、4%七氟醚组(SEV1、2组)和1.1%、2.2%异氟醚组(ISO1、2组)在37℃孵箱中分别暴露于2%、4%七氟醚和1.1%、2.2%异氟醚5 h,采用计算机辅助精子分析系统评价精子运动功能,记录精子运动活力[(a+b)%]、曲线速度(VCL)、直线速度(VSL)、平均速度(VAP)和头部侧向运动平均振幅(ALH),采用金霉素荧光染色技术评价精子体外获能情况.计算吸入麻醉药组各指标的相对抑制率.结果与C组比较,SEV1组、SEV2组和ISO2组(a+b)%、VCL、VSL、VAP降低,SEV2组ALH、SEV2组和ISO1、2组精子获能程度降低(P＜0.05);与SEV1组比较,SEV2组(a+b)%、VCL、VSL、VAP降低,ISO1组(a+b)%、VCL、VSL、VAP的相对抑制率降低(P＜0.05);与ISO1组比较,ISO2组(a+b)%、VCL、VSL、VAP降低(P＜0.05);与SEV2组比较,ISO2组(a+b)%、VCL、VSL、VAP和ALH的相对抑制率降低(P＜0.05).结论七氟醚和异氟醚均可呈剂量依赖性地抑制人精子运动功能和体外获能;七氟醚抑制精子运动功能的作用较异氟醚强.
목적 탐토불동흡입마취약대인정자운동공능화체외획능적영향.방법 성년남성정액경Percoll제도리심법처리후,침정정자치우인정자체외획능배양기,조절정자밀도(30～50)×106/ml,수궤분위5조(n=7):대조조(C조)정상배양,2%、4%칠불미조(SEV1、2조)화1.1%、2.2%이불미조(ISO1、2조)재37℃부상중분별폭로우2%、4%칠불미화1.1%、2.2%이불미5 h,채용계산궤보조정자분석계통평개정자운동공능,기록정자운동활력[(a+b)%]、곡선속도(VCL)、직선속도(VSL)、평균속도(VAP)화두부측향운동평균진폭(ALH),채용금매소형광염색기술평개정자체외획능정황.계산흡입마취약조각지표적상대억제솔.결과 여C조비교,SEV1조、SEV2조화ISO2조(a+b)%、VCL、VSL、VAP강저,SEV2조ALH、SEV2조화ISO1、2조정자획능정도강저(P＜0.05);여SEV1조비교,SEV2조(a+b)%、VCL、VSL、VAP강저,ISO1조(a+b)%、VCL、VSL、VAP적상대억제솔강저(P＜0.05);여ISO1조비교,ISO2조(a+b)%、VCL、VSL、VAP강저(P＜0.05);여SEV2조비교,ISO2조(a+b)%、VCL、VSL、VAP화ALH적상대억제솔강저(P＜0.05).결론 칠불미화이불미균가정제량의뢰성지억제인정자운동공능화체외획능;칠불미억제정자운동공능적작용교이불미강.
Objective To investigate the effects of inhalation anesthetics on human sperm motility and capacitation in vitro. Methods Sperm samples were obtained from normal adults and prepared with discontinuous percoll gradient centrifugation technique. The samples were incubated for 5 h in an airtight glass container filledwith 5% CO2-95% air at 37 ℃ with or without sevoflurane (SEV 2%, 4% ) or isoflurane (ISO 1.1%, 2.2% ).Then human sperm motility was examined in vitro at 37℃ and analyzed by the computer-assisted sperm analysis (CASA), including sperm motility (a + b)%, curvilinear velocity (VCL), straight line velocity (VSL), averagepath velocity (VAP) and amplitude of lateral head displacement (ALH). The capacitation effect was assessed by using the chlortetracycline (CTC) staining and phase-contract microscopy. Results 2% and 4% SEV significantly reduced (a + b)% , VCL, VSL and VAP in a dose-dependent manner, while only 4% SEV significantly decreased ALH and the capacitation ability of the sperm compared with control group. 2.2% ISO significantly decreased ( a + b)%, VCL, VSL and VAP compared with control and 1.1% ISO group. The capacitation ability of the sperm was significantly decreased by 1.1% and 2.2% ISO as compared with control group. Conclusion Sevoflurane and isoflurane have significant inhibitory effects on human sperm motility and capacitation in a dose-dependent manner. Sevoflurane has stronger inhibitory effect than isoflurane.