国际泌尿系统杂志
國際泌尿繫統雜誌
국제비뇨계통잡지
INTERNATIONAL JOURNAL OF UROLOGY AND NEPHROLOGY
2012年
2期
172-176
,共5页
宁雅娴%王俭勤%王雪婷%王雅%舒红%唐柏山
寧雅嫻%王儉勤%王雪婷%王雅%舒紅%唐柏山
저아한%왕검근%왕설정%왕아%서홍%당백산
肿瘤坏死因子%脐静脉%异黄酮
腫瘤壞死因子%臍靜脈%異黃酮
종류배사인자%제정맥%이황동
Tumor Necrosis Factor%Umbilical Veins%Isoflavones
目的 探讨黄芪单体毛蕊异黄酮对TNF-α诱导的人脐静脉内皮细胞(ECV-304)的一氧化氮(NO)、内皮素-1(ET-1)的影响.方法 用DMEM培养液在37℃、5％ CO2条件下对ECV304细胞株进行扩增至足够数量后,调细胞密度为1×105,并分为7组:A组:空白组,不加任何诱导剂；B组:TNF-α(40ng/ml)诱导组；C组:TNF-α(40ng/ml)+氯沙坦钾(105 mol/l)；D组:TNF -α(40ng/ml)+毛蕊异黄酮(0.1 mg/ml)；E组:TNF -α(40ng/ml)+毛蕊异黄酮(1mg/ml)；F组:TNF-α(40ng/ml)+毛蕊异黄酮(10mg/ml)；G组:TNF-α (40ng/ml)+毛蕊异黄酮(20mg/ml).分别于孵育6小时、24小时后时收集上清液,用ELISA检测上清液中一氧化氮(NO)、内皮素1(ET-1)的含量.结果 ①与空白组比较,TNF-α降低了ECV-304NO水平(P＜0.001)、提高了ET-1水平(P＜0.001)；②氯沙坦钾提高了ECV-304NO水平(P ＜0.001)、降低了ET-1水平(P＜0.001)；③毛蕊异黄酮(0.1 mg/ml～10mg/ml)能够促进ECV-304细胞NO的产生(P＜0.001)、降低ET -1的产生(P＜0.001)；④氯沙坦钾和毛蕊异黄酮(20mg/ml)对ECV-304细胞ET-1和NO水平的影响无明显差异((P＞0.05)；⑤毛蕊异黄酮浓度(在0.1 mg/ml ～20mg/ml内)与ECV-304 ET-1的分泌呈负相关性(r=-0.02、P＜0.001)；与ECV-304NO的分泌呈正相关性(r=0.0075、P ＜0.001).结论 黄芪毛蕊异黄酮单体可以促进TNF-α诱导的内皮细胞NO分泌,抑制ET-1分泌,并在一定浓度内(0.1 mg/ml～20mg/ml)呈浓度依赖性.
目的 探討黃芪單體毛蕊異黃酮對TNF-α誘導的人臍靜脈內皮細胞(ECV-304)的一氧化氮(NO)、內皮素-1(ET-1)的影響.方法 用DMEM培養液在37℃、5％ CO2條件下對ECV304細胞株進行擴增至足夠數量後,調細胞密度為1×105,併分為7組:A組:空白組,不加任何誘導劑；B組:TNF-α(40ng/ml)誘導組；C組:TNF-α(40ng/ml)+氯沙坦鉀(105 mol/l)；D組:TNF -α(40ng/ml)+毛蕊異黃酮(0.1 mg/ml)；E組:TNF -α(40ng/ml)+毛蕊異黃酮(1mg/ml)；F組:TNF-α(40ng/ml)+毛蕊異黃酮(10mg/ml)；G組:TNF-α (40ng/ml)+毛蕊異黃酮(20mg/ml).分彆于孵育6小時、24小時後時收集上清液,用ELISA檢測上清液中一氧化氮(NO)、內皮素1(ET-1)的含量.結果 ①與空白組比較,TNF-α降低瞭ECV-304NO水平(P＜0.001)、提高瞭ET-1水平(P＜0.001)；②氯沙坦鉀提高瞭ECV-304NO水平(P ＜0.001)、降低瞭ET-1水平(P＜0.001)；③毛蕊異黃酮(0.1 mg/ml～10mg/ml)能夠促進ECV-304細胞NO的產生(P＜0.001)、降低ET -1的產生(P＜0.001)；④氯沙坦鉀和毛蕊異黃酮(20mg/ml)對ECV-304細胞ET-1和NO水平的影響無明顯差異((P＞0.05)；⑤毛蕊異黃酮濃度(在0.1 mg/ml ～20mg/ml內)與ECV-304 ET-1的分泌呈負相關性(r=-0.02、P＜0.001)；與ECV-304NO的分泌呈正相關性(r=0.0075、P ＜0.001).結論 黃芪毛蕊異黃酮單體可以促進TNF-α誘導的內皮細胞NO分泌,抑製ET-1分泌,併在一定濃度內(0.1 mg/ml～20mg/ml)呈濃度依賴性.
목적 탐토황기단체모예이황동대TNF-α유도적인제정맥내피세포(ECV-304)적일양화담(NO)、내피소-1(ET-1)적영향.방법 용DMEM배양액재37℃、5％ CO2조건하대ECV304세포주진행확증지족구수량후,조세포밀도위1×105,병분위7조:A조:공백조,불가임하유도제；B조:TNF-α(40ng/ml)유도조；C조:TNF-α(40ng/ml)+록사탄갑(105 mol/l)；D조:TNF -α(40ng/ml)+모예이황동(0.1 mg/ml)；E조:TNF -α(40ng/ml)+모예이황동(1mg/ml)；F조:TNF-α(40ng/ml)+모예이황동(10mg/ml)；G조:TNF-α (40ng/ml)+모예이황동(20mg/ml).분별우부육6소시、24소시후시수집상청액,용ELISA검측상청액중일양화담(NO)、내피소1(ET-1)적함량.결과 ①여공백조비교,TNF-α강저료ECV-304NO수평(P＜0.001)、제고료ET-1수평(P＜0.001)；②록사탄갑제고료ECV-304NO수평(P ＜0.001)、강저료ET-1수평(P＜0.001)；③모예이황동(0.1 mg/ml～10mg/ml)능구촉진ECV-304세포NO적산생(P＜0.001)、강저ET -1적산생(P＜0.001)；④록사탄갑화모예이황동(20mg/ml)대ECV-304세포ET-1화NO수평적영향무명현차이((P＞0.05)；⑤모예이황동농도(재0.1 mg/ml ～20mg/ml내)여ECV-304 ET-1적분비정부상관성(r=-0.02、P＜0.001)；여ECV-304NO적분비정정상관성(r=0.0075、P ＜0.001).결론 황기모예이황동단체가이촉진TNF-α유도적내피세포NO분비,억제ET-1분비,병재일정농도내(0.1 mg/ml～20mg/ml)정농도의뢰성.
Objectives To investigate the astragalus calycosin monomer TNF - α induced human umbilical vein endothelial cells ( ECV - 304) of nitric oxide ( NO),endothelin - 1 ( ET - 1 ) effects.Methods DMEM culture medium at 37 ℃,5％ CO2 conditions on the ECV304 cell line was amplified to a sufficient number,the adjusted cell density of 1 × 105,and divided into 7 groups:A group:control group,without any inducer; B group:TNF-α (40ng/ml) induced group; C group:TNF-α (40ng/ml) + losastan (10 ～5mol/l) ; D group:TNF-α(40ng / ml) + calycosin (0.1mg/ml) ; E group:TNF - α (40ng/ml) + calycosin (lmg/ml) ; F group:TNF -α (40ng/ml) + calycosin (10mg/ml); G group:TNF-α (40ng/ml) + calycosin (20mg/ml).Were incubated for 6 hours,supernatant was collected after 24 hours,the supernatant by ELISA detection of nitric oxide (NO),endothelin 1 ( ET - 1 ) levels.Results ① compared with the control group,TNF - α reduced the ECV - 304 NO levels ( P ＜0.001 ),increased ET - 1 levels( P ＜ 0.001 ) ; ② losartan increased ECV - 304 NO levels( P ＜ 0.001),reduced the ET-1 levels( P ＜0.001) ; ③ calycosin (0.1 mg/ml～ 10mg/ml) ECV -304 cells can promote NO production( P ＜0.001 ),reduced ET- 1 production( P ＜ 0.001 ) ; ④ losartan potassium and calycosin (20mg/ml) ECV -304 cells for ET - 1 and NO levels not significantly different( P ＞ 0.05 ) ; ⑤ calycosin concentration (at 0.1 mg/ml -20mg/ml inside) and ECV-304 ET- 1 secretion was negatively correlated (r =- 0.02,P ＜ 0.001 ) ; and ECV -304 NO secretion was positively correlated (r =0.0075,P ＜0.001 ).Conc -lusions Astragalus calycosin monomer can promote TNF - α induced endothelial cell NO secretion,inhibition of ET - 1 secretion,and within a certain concentration (0,1 mg / ml ～ 20mg/ml) in a concentration dependent manner.
