中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2008年
3期
180-182
,共3页
胡晓芬%刘琴芝%李川%董婕%周剑芳%王敏%舒跃龙%刘宏图%梁米芳%李德新
鬍曉芬%劉琴芝%李川%董婕%週劍芳%王敏%舒躍龍%劉宏圖%樑米芳%李德新
호효분%류금지%리천%동첩%주검방%왕민%서약룡%류굉도%량미방%리덕신
正粘病毒科%流感病毒A型,鸟%表达的序列标记%芯片%聚合酶链反应
正粘病毒科%流感病毒A型,鳥%錶達的序列標記%芯片%聚閤酶鏈反應
정점병독과%류감병독A형,조%표체적서렬표기%심편%취합매련반응
Orthomyxo viridae%Influenza A virus,avian%Expressed sequence tags%Chip%Polymerase chain reaction
目的 筛选人细胞中与高致病性禽流感病毒致病相关的基因,探讨高致病性禽流感病毒的致病机理.方法 分别用高致病性禽流感病毒安徽株和普通人流感病毒H1N1株分别感染人肺癌上皮细胞,通过人类全基因表达谱芯片技术对不同时段感染细胞进行差异表达分析,筛选出与高致病性禽流感病毒感染相关的候选基因,以实时定量荧光PCR法验证差异表达基因.结果 获得了不同致病性流感病毒感染细胞后的差异表达谱,验证了细胞凋亡通路、mTOR通路中以及与免疫相关的16个基因的差异表达.结论 H5N1感染后与普通人流感病毒H1N1相比具有促进细胞凋亡的趋势.
目的 篩選人細胞中與高緻病性禽流感病毒緻病相關的基因,探討高緻病性禽流感病毒的緻病機理.方法 分彆用高緻病性禽流感病毒安徽株和普通人流感病毒H1N1株分彆感染人肺癌上皮細胞,通過人類全基因錶達譜芯片技術對不同時段感染細胞進行差異錶達分析,篩選齣與高緻病性禽流感病毒感染相關的候選基因,以實時定量熒光PCR法驗證差異錶達基因.結果 穫得瞭不同緻病性流感病毒感染細胞後的差異錶達譜,驗證瞭細胞凋亡通路、mTOR通路中以及與免疫相關的16箇基因的差異錶達.結論 H5N1感染後與普通人流感病毒H1N1相比具有促進細胞凋亡的趨勢.
목적 사선인세포중여고치병성금류감병독치병상관적기인,탐토고치병성금류감병독적치병궤리.방법 분별용고치병성금류감병독안휘주화보통인류감병독H1N1주분별감염인폐암상피세포,통과인류전기인표체보심편기술대불동시단감염세포진행차이표체분석,사선출여고치병성금류감병독감염상관적후선기인,이실시정량형광PCR법험증차이표체기인.결과 획득료불동치병성류감병독감염세포후적차이표체보,험증료세포조망통로、mTOR통로중이급여면역상관적16개기인적차이표체.결론 H5N1감염후여보통인류감병독H1N1상비구유촉진세포조망적추세.
Objective To identify genes in human cells infected with high pathogenic avian influenza viruses H5N1. Methods The lung carcinoma cells line A549 was infected with H5N1 and H1N1, respectively. We harvested the infected cells at the different time points after infection and screened the genes with differential expression via micro array technology. The candidate genes were selected and confirmed by quantitative real-time PCR. Results The spectrum of genes with the differential expression in the cells infected with H5N1 was obtained and 16 candidate genes were identified in the cellular apoptosis pathway, mTOR pathway, and the cellular immunity as well. Conclusion s Our Results suggest that H5N1 exert a stronger impact on eliciting apoptosis of infected cells than the common influenza virus H1N1.
