CAJ | 학술논문

目的观察X染色体开放读码框架6(CXorf6)在邻苯二甲酸二丁酯(DBP)致大鼠尿道下裂发生中的作用.方法于妊娠14～18 d(GD14～18)实验和对照组各10只孕鼠分别给予DBP 800 mg/(kg·d)和豆油2 ml/d灌胃,GD19剖宫产统计尿道下裂发生率,免疫印迹法和免疫组织化学法分析两组子鼠睾丸中CXorf6的表达.结果尿道下裂仅实验组发生,发生率为40.7％；CXorf6在尿道下裂组的相对表达量为0.323±0.014(n=10),对照组为0.706±0.016(n=10),差异有统计学意义(P＜0.05)；CXorf6主要定位于睾丸间质和支持细胞,尿道下裂组CXorf6着色细胞数量减少,染色强度弱于对照组.结论 DBP通过干预CXorf6的表达以影响睾丸的发育及雄激素的产生而导致尿道下裂的发生.
목적 관찰X염색체개방독마광가6(CXorf6)재린분이갑산이정지(DBP)치대서뇨도하렬발생중적작용.방법 우임신14～18 d(GD14～18)실험화대조조각10지잉서분별급여DBP 800 mg/(kg·d)화두유2 ml/d관위,GD19부궁산통계뇨도하렬발생솔,면역인적법화면역조직화학법분석량조자서고환중CXorf6적표체.결과 뇨도하렬부실험조발생,발생솔위40.7％；CXorf6재뇨도하렬조적상대표체량위0.323±0.014(n=10),대조조위0.706±0.016(n=10),차이유통계학의의(P＜0.05)；CXorf6주요정위우고환간질화지지세포,뇨도하렬조CXorf6착색세포수량감소,염색강도약우대조조.결론 DBP통과간예CXorf6적표체이영향고환적발육급웅격소적산생이도치뇨도하렬적발생.
Objective To verify the differential expression of X chromosome open reading frame 6 (CXorf6) in the testes of hypospadiac rats induced by maternal exposure to Di-n-butyl phthalate (DBP) and normal control rats in order to further explore the mechanism of hypospadias.Methods Twenty pregnant SD rats were randomly divided into two groups which were given DBP and soybean oil gavage at the dose of 800 mg/(kg·day) and 2 ml/day separately during the 14-18th day of pregnancy.Hypospadias incidence was calculated at GD19.The expression of CXorf6 was analyzed by Western blotting and immunohistochemistry.Results The incidence of hypospadias was 40.7％.There were statistically significant difference in the expression of CXorf6 between experiment group (0.323 ± 0.014) and control group (0.706 ±0.016,P ＜0.05).CXorf6 was mainly located in sertoli and leydig cells,and staining intensity in control group was stronger than in experimental group.Conclusion DBP could affect the occurrence of hypospadias by regnlating the expression of CXorf6 in fetal male rats.