中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2012年
1期
2-4
,共3页
谢晓丽%张伟%廖雪春%邓孝智%刘丽荣%张琪琛%商丽红%温燕%任敏
謝曉麗%張偉%廖雪春%鄧孝智%劉麗榮%張琪琛%商麗紅%溫燕%任敏
사효려%장위%료설춘%산효지%류려영%장기침%상려홍%온연%임민
腹泻,婴儿%轮状病毒属%流行病学,分子
腹瀉,嬰兒%輪狀病毒屬%流行病學,分子
복사,영인%륜상병독속%류행병학,분자
Diarrhea,infentile%Rotavirus%Epidemiology,molecular
目的 通过分子流行病学研究成都地区婴幼儿病毒性腹泻的病原学特点,掌握本地区病原分布特征,为疫苗研制和疫情控制提供科学依据.方法 采用酶联免疫吸附试验( ELISA)及逆转录聚合酶链反应(RT-PCR)对成都地区2006 -2008年度376例婴幼儿腹泻粪便标本进行轮状病毒(RV)、杯状病毒(HuCV)、星状病毒(AstV)及肠道腺病毒(Adv)检测.结果 RV的检出率为37.76％(142/376),其中42例进行G分型,45例进行P分型,G分型以G3型为主21例(50％),其次为G2和G1型,P分型以P[8]型为主21例,其次是P[4]型19例.RV感染主要为6～23月的婴幼儿,发病高峰在10 ～12月份(75.8％).RT-PCR法检出HuCV、AstV及Adv的检出率分别为15.85％、1.64％及2.04％.结论 RV是成都地区婴幼儿病毒性腹泻的主要病原,其流行的主要血清型为G3型、P[8]、P[4]型.除RV外,HuCV也是重要的病原.
目的 通過分子流行病學研究成都地區嬰幼兒病毒性腹瀉的病原學特點,掌握本地區病原分佈特徵,為疫苗研製和疫情控製提供科學依據.方法 採用酶聯免疫吸附試驗( ELISA)及逆轉錄聚閤酶鏈反應(RT-PCR)對成都地區2006 -2008年度376例嬰幼兒腹瀉糞便標本進行輪狀病毒(RV)、杯狀病毒(HuCV)、星狀病毒(AstV)及腸道腺病毒(Adv)檢測.結果 RV的檢齣率為37.76％(142/376),其中42例進行G分型,45例進行P分型,G分型以G3型為主21例(50％),其次為G2和G1型,P分型以P[8]型為主21例,其次是P[4]型19例.RV感染主要為6～23月的嬰幼兒,髮病高峰在10 ～12月份(75.8％).RT-PCR法檢齣HuCV、AstV及Adv的檢齣率分彆為15.85％、1.64％及2.04％.結論 RV是成都地區嬰幼兒病毒性腹瀉的主要病原,其流行的主要血清型為G3型、P[8]、P[4]型.除RV外,HuCV也是重要的病原.
목적 통과분자류행병학연구성도지구영유인병독성복사적병원학특점,장악본지구병원분포특정,위역묘연제화역정공제제공과학의거.방법 채용매련면역흡부시험( ELISA)급역전록취합매련반응(RT-PCR)대성도지구2006 -2008년도376례영유인복사분편표본진행륜상병독(RV)、배상병독(HuCV)、성상병독(AstV)급장도선병독(Adv)검측.결과 RV적검출솔위37.76％(142/376),기중42례진행G분형,45례진행P분형,G분형이G3형위주21례(50％),기차위G2화G1형,P분형이P[8]형위주21례,기차시P[4]형19례.RV감염주요위6～23월적영유인,발병고봉재10 ～12월빈(75.8％).RT-PCR법검출HuCV、AstV급Adv적검출솔분별위15.85％、1.64％급2.04％.결론 RV시성도지구영유인병독성복사적주요병원,기류행적주요혈청형위G3형、P[8]、P[4]형.제RV외,HuCV야시중요적병원.
Objective To investigated the molecular epidemiologic features of viral diarrhea in Chengdu infants and young children,and to establish baseline patterns of etiology,provides the scientific basis for the vaccine development and the epidemic situation control.Methods From March,2006 to December,2008,a total of 376 infants and young children from Chengdu area hospitalized for diarrhea in Chengdu Children's Hospital were enrolled in this study.The stool specimen collected from each patient was tested for rotavirus ( RV),Calicivirus ( CV),astrovirus ( AstV ) and adenovirus (Adv) by using enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) examination.Results Among those 376 cases,there were 142 cases (37.76％) of RV infections,which scattered predominantly in October to December.Among 234 cases RV negativity,there were 29 cases HuCV infections ( 15.85％ ),5 cases AstV infections ( 1.64％ ),and 8 cases Adv infections (2.04％).Conclusion RV appeared to be the main etiological agent of viral diarrhea in Chengdu infants and young children,the predominant serotype of RV were G3,P[ 8] and P [ 4 ],HuCV might be the important etiological agent besides RV.
