CAJ | 학술논문

作为植物有性繁殖器官--花的花瓣通常生命周期短,其中有一个敏感的、严格控制的细胞程序化死亡过程.为了揭示细胞程序化死亡过程中发生的反应或者其组成成分,解释玫瑰花发育过程中的细胞程序化死亡过程的机理,测定了在整个花发育过程中玫瑰花瓣的乙烯释放速率、ACC合酶基因的转录产物(mRNA)、ACC合酶活性以及ACC含量.结果显示在花发育过程前期(阶段1、2)检测不到乙烯产生,在花瓣完全绽开时花瓣中乙烯开始产生.在花发育后期(阶段4、5)花的衰老与乙烯释放速率的升高同时发生.在花发育前期没有ACC合酶基因的转录产物积累,该基因在花瓣完全绽开时开始表达,在花发育后期逐渐增强.ACC合酶活性与ACC含量的变化趋势与乙烯的一致.在玫瑰花发育后期乙烯诱导和调控花瓣的细胞程序化死亡.ACC合酶基因、ACC合酶以及ACC都是玫瑰花瓣程序化死亡过程中的重要调控因子.
작위식물유성번식기관--화적화판통상생명주기단,기중유일개민감적、엄격공제적세포정서화사망과정.위료게시세포정서화사망과정중발생적반응혹자기조성성분,해석매괴화발육과정중적세포정서화사망과정적궤리,측정료재정개화발육과정중매괴화판적을희석방속솔、ACC합매기인적전록산물(mRNA)、ACC합매활성이급ACC함량.결과현시재화발육과정전기(계단1、2)검측불도을희산생,재화판완전탄개시화판중을희개시산생.재화발육후기(계단4、5)화적쇠로여을희석방속솔적승고동시발생.재화발육전기몰유ACC합매기인적전록산물적루,해기인재화판완전탄개시개시표체,재화발육후기축점증강.ACC합매활성여ACC함량적변화추세여을희적일치.재매괴화발육후기을희유도화조공화판적세포정서화사망.ACC합매기인、ACC합매이급ACC도시매괴화판정서화사망과정중적중요조공인자.
Programmed cell death (PCD) is an integral part of plant development. Flower petal usually has the shortest lifetime among all plant organs. There must be a sensitive, tightly controlled PCD in the life cycle of the flower. To understand its mechanism, the ethylene production rate of petals and its correlation with degree of senescence, 1-aminocyclopropane-1carboxylate (ACC) synthase gene expression, ACC synthase activity and ACC content were determined through the whole flower development period which was arbitrarily divided into five stages depending on appearance of the flower. The results showed that ethylene was not detectable at stages 1 and 2, appeared at stage 3 and increased at stage 5. Transcript of ACC synthase gene did not accumulate at stages 1 and 2, but did so at stages 3-5, and increased gradually at stage 5. ACC synthase activity and ACC content changed in similar way to ethylene production. Ethylene plays a critical role in initiation of rose flower senescence through regulating petal PCD.