时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2008年
6期
1544-1546
,共3页
周英%郭白苏%郑燕%黄赤夫
週英%郭白囌%鄭燕%黃赤伕
주영%곽백소%정연%황적부
罗汉果果实%HPLC组分%抗茵活性%罗汉果苷
囉漢果果實%HPLC組分%抗茵活性%囉漢果苷
라한과과실%HPLC조분%항인활성%라한과감
Momordica grosvenori%HPLC fractionation%Antibacterial activity%Mogrosides
目的 确定并纯化罗汉果果实中的抑茵组分.方法 制备罗汉果干果的粗提物,并通过一种快速的颜色指示法检测其抗菌活性.罗汉果果实提取物对变形链球菌(S.mutans)有很强的抑制活性.为了进一步分离活性组分及成分,通过HPLC对其进行分离,并通过上述筛选方法对不同组分的抑茵活性进行快速筛选,对有活性的组分通过血琼脂法进行验证.结果 罗汉果果实提取物对变形链球菌(S.mutans)有很强的抑制活性;将其粗提物通过HPLC进行分离得到50个组分,罗汉果果实提取物的两个HPLC组分,#18-19和#34-35对于变形链球茵(S.mutans)有很强的抑制活性,而其它的组分没有或只有很弱的活性.罗汉果苷V无抑茵活性.结论 罗汉果果实提取物具有较强的抑茵活性;罗汉果果实的抑茵活性不是由罗汉果苷,而是由罗汉果中另外的活性组分或成分产生的.罗汉果果实具有进一步开发为抗茵药物和保健品的潜力.
目的 確定併純化囉漢果果實中的抑茵組分.方法 製備囉漢果榦果的粗提物,併通過一種快速的顏色指示法檢測其抗菌活性.囉漢果果實提取物對變形鏈毬菌(S.mutans)有很彊的抑製活性.為瞭進一步分離活性組分及成分,通過HPLC對其進行分離,併通過上述篩選方法對不同組分的抑茵活性進行快速篩選,對有活性的組分通過血瓊脂法進行驗證.結果 囉漢果果實提取物對變形鏈毬菌(S.mutans)有很彊的抑製活性;將其粗提物通過HPLC進行分離得到50箇組分,囉漢果果實提取物的兩箇HPLC組分,#18-19和#34-35對于變形鏈毬茵(S.mutans)有很彊的抑製活性,而其它的組分沒有或隻有很弱的活性.囉漢果苷V無抑茵活性.結論 囉漢果果實提取物具有較彊的抑茵活性;囉漢果果實的抑茵活性不是由囉漢果苷,而是由囉漢果中另外的活性組分或成分產生的.囉漢果果實具有進一步開髮為抗茵藥物和保健品的潛力.
목적 학정병순화라한과과실중적억인조분.방법 제비라한과간과적조제물,병통과일충쾌속적안색지시법검측기항균활성.라한과과실제취물대변형련구균(S.mutans)유흔강적억제활성.위료진일보분리활성조분급성분,통과HPLC대기진행분리,병통과상술사선방법대불동조분적억인활성진행쾌속사선,대유활성적조분통과혈경지법진행험증.결과 라한과과실제취물대변형련구균(S.mutans)유흔강적억제활성;장기조제물통과HPLC진행분리득도50개조분,라한과과실제취물적량개HPLC조분,#18-19화#34-35대우변형련구인(S.mutans)유흔강적억제활성,이기타적조분몰유혹지유흔약적활성.라한과감V무억인활성.결론 라한과과실제취물구유교강적억인활성;라한과과실적억인활성불시유라한과감,이시유라한과중령외적활성조분혹성분산생적.라한과과실구유진일보개발위항인약물화보건품적잠력.
Objective This research intended to study and identify the antibacterial activitt of Lo Han Kuo fruit.Methods The fruit extract was prepared from dry Lo Han Kuo fruit and then screened for antibacterial activity using a colorimetric detection method.To further iselate the bioactive fractions,the fruit extract was separated into smaller fractions Via higll-performance liquid chromatography(HPLC)and the fractions were tested again for antibacterial activity.The positive fractions showing antibacterial activity were further tested by blood agar plating method.Results Lo Han Kuo fruit extract exhibited strong antibacterial activity against the oral bacteria,S.mutans,After seperated by HPLC,two major HPLC fractions of Lo Hart Kuo fruit extract.fraction 18～19 and 34～35,demonstrated significant inhibition for oral bacteria S.mutarts,while other fractions exhibited some,but not complete inhibition.Furthermore,the antibacterial activity seems to be not related with mogrosides because the pure mogroside V exhibited no antibacterial activity.Conclusion This is the first study demonstrating that Lo Han Kuo fruit extract exhibited strong antibacterial activity but not due to the presence of mogrosides.
