CAJ | 학술논문

MiR396是一个由21个核苷酸组成的单链非编码RNA小分子.烟草内的miR396受干旱诱导说明其可能参与烟草的干旱应答.在35S强启动子作用下我们将miR396转入到烟草体内获得高表达转基因植株,生理学测试表明高表达miR396的转基因烟草耐旱性增强,同时叶片表现出比野生型较低的失水率和较高的相对含水量,进一步分析表明转基因植株除了叶片变得更为窄小外,其气孔密度和气孔系数都比野生型降低,这些都表明miR396作为一个正调节因子参与烟草的干旱胁迫应答.
MiR396시일개유21개핵감산조성적단련비편마RNA소분자.연초내적miR396수간한유도설명기가능삼여연초적간한응답.재35S강계동자작용하아문장miR396전입도연초체내획득고표체전기인식주,생이학측시표명고표체miR396적전기인연초내한성증강,동시협편표현출비야생형교저적실수솔화교고적상대함수량,진일보분석표명전기인식주제료협편변득경위착소외,기기공밀도화기공계수도비야생형강저,저사도표명miR396작위일개정조절인자삼여연초적간한협박응답.
MiR396 was a single-stranded noncoding small RNA with 21 nucleotides, and the expression of MiR369 in leaves was strongly induced in water deficit condition in tobacco, which suggested a possible role of miR396 in drought stress response. Under the control of 35S promoter, MIR396 was introduced into tobacco mediated by Agrobacterium tumefaciens. Physiological tests indicated that the elevated levels of miR396 increased drought tolerance in tobacco accompanying with lower water loss rate and higher relative water content. Further more miR396-overexpressing plants exhibited visible reductions both in stomatal density and stomatal index as well as a narrow and small leaf phenotype in comparison with wild-type plants. The present study indicated that miR396 was a positive regulator in response to drought stress in tobacco.