复旦学报（医学版）
複旦學報（醫學版）
복단학보（의학판）
FUDAN UNIVERSITY JOURNAL OF MEDICAL SCIENCES
2009年
6期
675-680
,共6页
王莉%杨永杰%归绥琪%陈松华%葛锡锐%李桂玲
王莉%楊永傑%歸綏琪%陳鬆華%葛錫銳%李桂玲
왕리%양영걸%귀수기%진송화%갈석예%리계령
掌叶半夏%β-谷甾醇%子宫颈癌%细胞周期
掌葉半夏%β-穀甾醇%子宮頸癌%細胞週期
장협반하%β-곡치순%자궁경암%세포주기
Pinella%β-sitosterol%cervical cancer%cell cycle
目的 观察掌叶半夏主要成分对子宫颈癌细胞活性、形态和超微结构、细胞周期和凋亡的影响.方法 在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和/或掌叶半夏总蛋白培养1～7 d,采用MTT比色法检测细胞的活性.采用倒置相差显微镜、扫描和透射电镜观察细胞形态学和超微结构的变化,流式细胞术检测β-谷甾醇对细胞周期和细胞凋亡的影响.结果 β-谷甾醇对子宫颈癌细胞SiHa的活性表现出明显的抑制作用,且具有时间、剂量依赖关系,掌叶半夏总蛋白对SiHa细胞的活性无显著影响.与对照组比较,20 μmol/L的β-谷甾醇使SiHa细胞出现S期聚集、凋亡和坏死细胞增加,细胞形态和超微结构发生显著改变.结论 掌叶半夏总蛋白对SiHa细胞活性的抑制作用不明显,β-谷甾醇能抑制人子宫颈癌细胞的活性,使细胞周期聚集在S期,诱导少量细胞凋亡和坏死,对细胞的形态和超微结构有显著的影响.因此,β-谷甾醇有希望成为一种安全低毒的抗子宫颈癌药物.
目的 觀察掌葉半夏主要成分對子宮頸癌細胞活性、形態和超微結構、細胞週期和凋亡的影響.方法 在子宮頸癌細胞SiHa的培養基中加入不同濃度的β-穀甾醇和/或掌葉半夏總蛋白培養1～7 d,採用MTT比色法檢測細胞的活性.採用倒置相差顯微鏡、掃描和透射電鏡觀察細胞形態學和超微結構的變化,流式細胞術檢測β-穀甾醇對細胞週期和細胞凋亡的影響.結果 β-穀甾醇對子宮頸癌細胞SiHa的活性錶現齣明顯的抑製作用,且具有時間、劑量依賴關繫,掌葉半夏總蛋白對SiHa細胞的活性無顯著影響.與對照組比較,20 μmol/L的β-穀甾醇使SiHa細胞齣現S期聚集、凋亡和壞死細胞增加,細胞形態和超微結構髮生顯著改變.結論 掌葉半夏總蛋白對SiHa細胞活性的抑製作用不明顯,β-穀甾醇能抑製人子宮頸癌細胞的活性,使細胞週期聚集在S期,誘導少量細胞凋亡和壞死,對細胞的形態和超微結構有顯著的影響.因此,β-穀甾醇有希望成為一種安全低毒的抗子宮頸癌藥物.
목적 관찰장협반하주요성분대자궁경암세포활성、형태화초미결구、세포주기화조망적영향.방법 재자궁경암세포SiHa적배양기중가입불동농도적β-곡치순화/혹장협반하총단백배양1～7 d,채용MTT비색법검측세포적활성.채용도치상차현미경、소묘화투사전경관찰세포형태학화초미결구적변화,류식세포술검측β-곡치순대세포주기화세포조망적영향.결과 β-곡치순대자궁경암세포SiHa적활성표현출명현적억제작용,차구유시간、제량의뢰관계,장협반하총단백대SiHa세포적활성무현저영향.여대조조비교,20 μmol/L적β-곡치순사SiHa세포출현S기취집、조망화배사세포증가,세포형태화초미결구발생현저개변.결론 장협반하총단백대SiHa세포활성적억제작용불명현,β-곡치순능억제인자궁경암세포적활성,사세포주기취집재S기,유도소량세포조망화배사,대세포적형태화초미결구유현저적영향.인차,β-곡치순유희망성위일충안전저독적항자궁경암약물.
Objective To investigate the effects of Pinella's ingredients on the viability of cells, morphology, microstructure, cell cycle and apoptosis in human cervical cancer cell lines. Methods The β-sitosterol and/or total protein of Pinella were incubated at different concentrations with cervical cancer cell line SiHa. The effects of β-sitosterol and/or total protein of Pinella on the viability of cells were tested by MTT assay. The effects of β-sitosterol on morphology, microstructure, cell cycle and apoptosis were studied by phase-contrast microscope, electron microscope and flowcytometry, respectively. Results β-sitosterol could obviously inhibit the viability of SiHa cells in a time-and dose-dependent manner. The total protein of Pinella had no effects on SiHa cells' viability. 20 μmol/L β-sitosterol induced the accumulation of SiHa cells in S phase in the cell cycle. And the percents of apoptosis and necrosis increased. The morphology and microstructure of SiHa cells changed significantly after treated with 20 μmol/L β-sitosterol. Conclusions The total protein of Pinella had little influence on the viability of cervical cancer cells SiHa. The viability of SiHa cells could be suppressed by β-sitosterol. β-sitosterol could induce the accumulation of cells in S phase and the percent of apoptosis and necrosis. The morphology and microstructure changed significantly after treated with β-sitosterol. Therefore, β-sitosterol might be a prospect safe and low toxicity anti-cervical cancer drug.
